大鼠不同成熟状态骨髓源树突状细胞的培养与鉴定

被引：6

作者：

李春满

付必莽

谢楠

董丽英

张理超

赵越

胡明道

张捷

机构：

[1] 昆明医科大学第二附属医院肝胆胰外科

来源：

云南大学学报(自然科学版) | 2016年 / 38卷 / 02期

关键词：

树突细胞; 不同成熟状态; 体外细胞培养; 鉴定;

D O I：

暂无

中图分类号：

R329.24 [];

学科分类号：

摘要：

分离、纯化大鼠骨髓单个核细胞,在培养体系中添加重组大鼠粒-巨噬细胞集落刺激因子(rrGMCSF)和重组大鼠白细胞介素(rrIL-4)刺激获得未成熟树突状细胞(imDC),再分别以肿瘤坏死因子-α(TNF-α)和脂多糖(LPS)刺激获得半成熟DC(smDC)和成熟DC(mDC).并对imDC、smDC和mDC的形态、表型、细胞因子分泌情况进行鉴定,检测不同DC对异基因淋巴细胞的激活功能.检测结果符合文献对DC的介定,培养所得为imDC、smDC和mDC.可见,体外利用GM-CSF、IL-4、TNF-α和LPS诱导骨髓单核细胞是获得大鼠3种不同成熟DCs的有效方法.

引用

页码：317 / 325

页数：9

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