基于石墨烯-纳米金-丝素水凝胶的高灵敏有机相酶电极检测呋喃丹

被引：1

作者：

许绍鹏 ^{[1
]}

董静 ^{[2
]}

艾仕云 ^{[2
]}

机构：

[1] 山东省沾化县冯家镇第六小学

[2] 山东农业大学化学与材料科学学院

来源：

化学传感器 | 2013年 / 33卷 / 03期

关键词：

石墨烯; 酪氨酸酶; 纳米金; 有机相酶电极; 呋喃丹;

D O I：

暂无

中图分类号：

O657.1 [电化学分析法]; TQ450.7 [产品分析及检验];

学科分类号：

摘要：

采用改进的Hummers法制备了氧化石墨烯(GO),用微波辅助丝素还原法制备了丝素还原石墨烯(SF-GR),并用红外(FT-IR)、紫外(UV-vis)和透射电镜(TEM)对SF-GR进行了表征。然后以丝素同时原位还原的石墨烯-纳米金复合水凝胶包埋酪氨酸酶(Tyr)制备了新型的有机相酶电极(SF-GR-NanoAu-Tyr/GCE),在纯氯仿中对呋喃丹进行了检测。结果表明,在有机相中,丝素水凝胶能较好的保持酪氨酸酶的生物活性,GR和NanoAu促进了电子在电极界面上以及水凝胶内部的电子传递,提高了酶电极的灵敏性。在没有另外添加水或者缓冲液的情况下,酪氨酸酶的抑制率与浓度范围为1.0×10-8～1.0×10-12mol/L的呋喃丹呈线性关系,检测限为8.0×10-13mol/L。该有机相酶电极制备简单,检测快速,灵敏度高,适合于有机溶剂中微量农药的定量检测。

引用

页码：35 / 41

页数：7

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