影响花椰菜下胚轴原生质体培养和植株再生的因素

被引：11

作者：

卫志明

许智宏

机构：

[1] 中国科学院上海植物生理研究所,中国科学院上海植物生理研究所上海,上海

来源：

植物生理学报 | 1990年 / 04期

关键词：

花椰菜; 下胚轴; 原生质体培养; 再生植株;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

从花椰菜的无菌苗下胚轴游离原生质体经纯化后的得率为1.5～2×10～6/g FW。通过液体浅层培养、平板固体培养、双层培养和gelrlte包埋培养方法的比较,发现gelrite包埋培养法,最有利于花椰菜下胚轴的原生质体培养。纯化的原生质体用MS-1培养基培养,再生细胞的分裂频率为24％。再生的愈伤组织转到分化培养基MS-5A或MS-5B上迅速分化出苗。共获得再生植株78株,移栽到盆中生长正常,结出正常的菜花。

引用

页码：394 / 400

页数：7

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