钙-钙调素对小麦hsp26基因表达的影响

被引:7
作者
刘宏涛
赵和
李冰
孙大业
周人纲
机构
[1] 河北省农林科学院农业物理生理生化研究所!石家庄河北师范大学生命科学学院,石家庄,河北省农林科学院农业物理生理生化研究所!石家庄,河北省农林科学院农业物理生理生化研究所!石家庄河北师范大学生命科学学院,石家庄,河北师范大学生命科学学院!
关键词
热激蛋白; 钙-钙调素; 基因表达; 小麦;
D O I
暂无
中图分类号
Q943.2 [植物基因工程];
学科分类号
071007 ; 090102 ;
摘要
The treatment of wheat ( Triticum aestivum L.) seedlings with CaCl 2 increased mRNA levels of hsp26 gene at 37 ℃ for 1 h, while that with Ca 2+ chelator EGTA decreased the expression of the hsp26 gene. The expression of the wheat calmodulin gene CaM12 is rapidly upregulated in wheat seedlings by heat shock at 37 ℃. The heatinduced upregulation of CaM12 occurred earlier than that of the hsp26 gene did. Calmodulin antagonist N6aminohexyl5chloro1_naphthalenesulfonamid (W7) inhibited the expression of the hsp26 gene in wheat seedlings at 37 ℃ for 1 h. These results implied that the calciumcalmodulin is probably involved in the signal transduction of the hsp26 gene expression induced by heat shock.
引用
收藏
页码:766 / 768
页数:3
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[2]  
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