利用ISSR标记进行菜用大豆和普通大豆的分类(英文)

被引：4

作者：

谢甫绨 ^{[1
]}

Takahata Yoshihito ^{[2
]}

Ohno Yasuo ^{[3
]}

机构：

[1] 沈阳农业大学农学院

[2] Faculty of Agriculture,Iwate University,-,Japan

[3] Sato Masayuki Seed Co,Yahaba-cho-,Japan

来源：

大豆科学 | 2008年 / 05期

关键词：

大豆; ISSR标记; 多样性;

D O I：

暂无

中图分类号：

S565.1 [大豆];

学科分类号：

0901 ;

摘要：

ISSR标记技术因具有很高的多肽性而被广泛应用于遗传多样性研究。进行菜用大豆品种多样性研究将有助于育种家进行杂交亲本的合理选配。采用ISSR标记技术对37个菜用大豆品种(系)和13个普通大豆品种(系)进行了分类研究。结果表明:50个ISSR引物中有11个引物没有多肽性,其他39个引物共产生132条谱带,其中81条多肽性谱带,占61.4%。引物扩增的谱带数在1～7条之间,大小为830～3530 bp.每个引物平均扩增的谱带数和多肽性带数分别为3.3和2.1。聚类分析结果表明,可将供试的50个品种划分为3个类组,扁茎大豆Taikadai-zu单独一组,7个普通大豆(多数为中国选育)划归为B组,C组由日本菜用大豆和4个普通大豆组成。C组还可以划分成若干亚组,同一亚组的品种具有相似的性状,比如有一亚组的品种均是褐脐、茶色种皮、白花、不易炸荚。系选的品种和原始品种在分类图中也显示出其密切的亲缘关系。基于ISSR标记的分类可以反映菜用大豆的遗传关系,菜用大豆和普通大豆之间存在遗传多样性,合理保存菜用大豆种质将有助于未来大豆品质改良计划。

引用

页码：732 / 739

页数：8

共 11 条

[1]

Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding[J] . M. Pradeep Reddy,N. Sarla,E.A. Siddiq.Euphytica . 2002 (1)

[2]

Genetic variation within and among populations of a wild rice Oryza granulata from China detected by RAPD and ISSR markers[J] . W. Qian,S. Ge,D.-Y. Hong.TAG Theoretical and Applied Genetics . 2001 (2-3)

[3]

Evaluation of inter-simple sequence repeat analysis for mapping in Citrus and extension of the genetic linkage map[J] . A. A. Sankar,G. A. Moore.TAG Theoretical and Applied Genetics . 2001 (2-3)

[4] Inter simple sequence repeat analysis of genetic relationships in the genus Vigna [J].

Ajibade, SR ;

Weeden, NF ;

Chite, SM .

EUPHYTICA, 2000, 111 (01) :47-55

[5]

A comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR) in tetraploid potato ( Solanum tuberosum L.) germplasm[J] . C.E. McGregor,C.A. Lambert,M.M. Greyling,J.H. Louw,L. Warnich.Euphytica . 2000 (2)

[6] Genetic diversity and phylogenetic relationship as revealed by inter simple sequence repeat (ISSR) polymorphism in the genus Oryza [J].

Joshi, SP ;

Gupta, VS ;

Aggarwal, RK ;

Ranjekar, PK ;

Brar, DS .

THEORETICAL AND APPLIED GENETICS, 2000, 100 (08) :1311-1320

[7] Inter-simple sequence repeats PCR for characterization of closely related grapevine germplasm [J].

Moreno, S ;

Martín, JP ;

Ortiz, JM .

EUPHYTICA, 1998, 101 (01) :117-125

[8]

Genetic variance, coefficient of parentage, and genetic distance of six soybean populations[J] . T. Helms,G. Vallad,P. McClean,J. Orf.Theoretical and Applied Genetics . 1997 (1)

[9]

Assessment of genetic diversity in dent and popcorn ( Zea mays L.) inbred lines using inter-simple sequence repeat (ISSR) amplification[J] . Ramesh V. Kantety,Xiaoping Zeng,Jeffrey L. Bennetzen,Brent E. Zehr.Molecular Breeding . 1995 (4)

[10]

Evaluation of soybean RFLP marker diversity in adapted germ plasm[J] . P. Keim,W. Beavis,J. Schupp,R. Freestone.Theoretical and Applied Genetics . 1992 (2)

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