Wolbachia在Drosophila auraria复合种和Drosophila simulans中的感染

被引：3

作者：

周伟国

赵新燕

王蓉

甘波谊

李昌本

机构：

[1] 复旦大学遗传工程国家重点实验室

来源：

复旦学报(自然科学版) | 1999年 / 04期

关键词：

Wolbachia; Drosophilaauraria复合种; wsp基因; 细胞色素氧化酶2亚基基因;

D O I：

10.15943/j.cnki.fdxb-jns.1999.04.011

中图分类号：

Q933 [微生物遗传学];

学科分类号：

071007 ;

摘要：

用ＰＣＲ方法检测了多种果蝇中共生菌Ｗｏｌｂａｃｈｉａ的感染，发现Ｄｒｏｓｏｐｈｉｌａａｕｒａｒｉａ复合种以及采集于北京地区的Ｄｒｏｓｏｐｈｉｌａｓｉｍｕｌａｎｓ为Ｗｏｌｂａｃｈｉａ所感染，ＲＦＬＰ分析证实为单一感染．克隆了编码Ｗｏｌｂａｃｈｉａ外膜蛋白质的ｗｓｐ基因并进行了序列测定．同时比较了Ｄｒｏｓｏｐｈｉｌａａｕｒａｒｉａ复合种内４个种和采集于北京及美国加州的Ｄｒｏｓｏｐｈｉｌａｓｉｍｕｌａｎｓ的线粒体的细胞色素氧化酶２亚基基因的部分序列．进而讨论了Ｗｏｌｂａｃｈｉａ对Ｄｒｏｓｏｐｈｉｌａａｕｒａｒｉａ复合种成员之间进化关系的影响．

引用

页码：428 / 427

页数：7

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