南海南部陆坡表层沉积物细菌和古菌多样性

被引：38

作者：

李涛

王鹏

汪品先

机构：

[1] 同济大学海洋地质国家重点实验室

来源：

微生物学报 | 2008年 / 03期

关键词：

南海; 沉积物; 16SrDNA; 多样性;

D O I：

10.13343/j.cnki.wsxb.2008.03.011

中图分类号：

Q913 [微体古生物学];

学科分类号：

0709 ; 070903 ;

摘要：

从南海南部陆坡表层沉积物中扩增了细菌和古菌16S rDNA序列,并对克隆子文库进行系统发育分析。细菌序列以变形杆菌(Proteobacteria)居多,其次是浮霉菌(Planctomycete)、酸杆菌(Acidobacteria)和candidate division OP10,另外还有少量铁还原杆菌(Deferrobacteres)、candidate di-vision OP3、OP11、OP8、TM6、疣微菌(Verrucomicrobia)和螺旋体(Spirochaetes)。古菌序列分别来自泉古生菌(Crenarchaeota)和广古生菌(Euryarchaeota),以Marine Benthic Group B(MBGB)、Marine Crenarchaeotic GroupⅠ(MGⅠ)、Marine Benthic Group D(MBGD)和South African Gold Mine Eu-ryarchaeotic Group(SAGMEG)为主。少量序列为C3、甲烷杆菌(Methanobacteriales)和Novel Eu-ryarchaeotic Group(NEG)。结果表明海底表层沉积物中有丰富多样的微生物群落。

引用

页码：323 / 329

页数：7

共 7 条

[1]

Prokaryotic community composition and biogeochemical processes in deep subseafloor sediments from the Peru Margin[J] . Gordon Webster,R. John Parkes,Barry A. Cragg,Carole J. Newberry,Andrew J. Weightman,John C. Fry.FEMS Microbiology Ecology . 2006 (1)

[2] Bacterial diversity in hydrothermal sediment and epsilon proteobacterial dominance in experimental microcolonizers at the Mid-Atlantic Ridge [J].

López-García, P ;

Duperron, S ;

Philippot, P ;

Foriel, J ;

Susini, J ;

Moreira, D .

ENVIRONMENTAL MICROBIOLOGY, 2003, 5 (10) :961-976

[3] Microbial diversity in sediments associated with surface-breaching gas hydrate mounds in the Gulf of Mexico [J].

Mills, HJ ;

Hodges, C ;

Wilson, K ;

MacDonald, IR ;

Sobecky, PA .

FEMS MICROBIOLOGY ECOLOGY, 2003, 46 (01) :39-52

[4]

Assessment of bacterial community structure in the deep sub-seafloor biosphere by 16S rDNA-based techniques: a cautionary tale[J] . Gordon Webster,Carole J. Newberry,John C. Fry,Andrew J. Weightman.Journal of Microbiological Methods . 2003 (1)

[5]

Recent studies on bacterial populations and processes in subseafloor sediments: A review[J] . R. John Parkes,Barry A. Cragg,Peter Wellsbury.Hydrogeology Journal . 2000 (1)

[6]

Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis[J] . FEMS Microbiology Reviews . 1997 (3)

[7]

A molecular approach to search for diversity among bacteria in the environment[J] . H Rheims,F A Rainey,E Stackebrandt.Journal of Industrial Microbiology ＆ Biotechnology . 1996 (3)

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