葛花鸢尾苷的酸水解及其对乙醇脱氢酶激活作用的研究

被引：10

作者：

胡玉涛 ^{[1
]}

马双双 ^{[2
]}

刘莹 ^{[2
]}

王益 ^{[2
]}

黄文 ^{[2
]}

机构：

[1] 江苏联合职业技术学院连云港中医药分院

[2] 华中农业大学食品科学技术学院

来源：

天然产物研究与开发 | 2013年 / 25卷 / 09期

关键词：

葛花; 鸢尾苷; 鸢尾苷元; 酸水解; 乙醇脱氢酶;

D O I：

10.16333/j.1001-6880.2013.09.023

中图分类号：

TQ461 [中草药制剂的生产]; R284.3 [有效成分的化学合成];

学科分类号：

091003 [林草生态工程学]; 100804 [中药化学];

摘要：

本文对葛花提取物鸢尾苷经酸解法得到鸢尾苷元的水解工艺及鸢尾苷和鸢尾苷元对乙醇脱氢酶的激活作用进行了研究。结果表明,葛花鸢尾苷酸水解的最佳条件为:固液比1∶1(mg/mL),甲醇浓度50%,反应温度80℃,反应时间5 h,盐酸浓度4%,此条件下,鸢尾苷水解率可达99%以上。鸢尾苷和鸢尾苷元对乙醇脱氢酶均有一定的激活作用,其激活率分别为127.35%和132.35%。酸水解提高了葛花异黄酮类物质的生物利用率。

引用

页码：1266 / 1270

页数：5

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