裸燕麦麸β-葡聚糖的提纯分离及结构研究

被引：13

作者：

申瑞玲

姚惠源

机构：

[1] 江南大学食品学院

来源：

郑州工程学院学报 | 2004年 / 04期

关键词：

裸燕麦麸; β-葡聚糖; 纯化; 结构;

D O I：

10.16433/j.cnki.issn1673-2383.2004.04.011

中图分类号：

TS210.2 [原粮];

学科分类号：

摘要：

从裸燕麦麸皮中提取β 葡聚糖,用硫酸铵沉淀法对粗提物进行初步纯化得到组分POG,利用离子交换柱色谱和SepharoseCL-4B凝胶柱色谱对POG进一步纯化得到组分POG11和POG12,用气相色谱测定了它们的单糖组成,并经荧光吸收表明组分POG11为β 葡聚糖,用酶法测定其β 葡聚糖含量为98.6%,高效液相色谱显示该组分相对分子质量为2.62×106,红外光谱和13C核磁共振表明POG11是混合键(1→3)(1→4)-β-D-葡聚糖.

引用

页码：44 / 47

页数：4

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