缺血后处理联合水蛭注射液对脑缺血损伤大鼠PI3K表达的影响

被引：13

作者：

胡跃强 ^{[1
]}

兰鹏 ^{[2
]}

秦红玲 ^{[1
]}

苏锦勋 ^{[2
]}

覃琴 ^{[1
]}

机构：

[1] 广西中医药大学第一附属医院

[2] 广西南宁市中医医院

来源：

中西医结合心脑血管病杂志 | 2018年 / 16卷 / 02期

关键词：

脑缺后损伤; 缺血后处理; 水蛭注射液; 磷脂酸肌醇-3激酶;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

100806 [中药药理学];

摘要：

目的观察缺血后处理(IPOC)联合水蛭注射液对脑缺血损伤大鼠磷脂酸肌醇-3激酶(PI3K)mRNA及其蛋白表达和脑梗死体积的影响。方法 SD大鼠40只,随机分为假手术组(SO组)、缺血再灌注(MCAO)组、缺血后处理组(IPOC组)、缺血后处理+水蛭注射液后处理组(P-L组)4组。采用线栓法制备大鼠IPOC和MCAO模型,采用TTC染色法计算脑梗死体积,实时荧光定量PCR和Western blot技术检测PI3K mRNA及其蛋白的表达变化。结果大鼠脑缺血再灌注后24h,IPOC组较MCAO组梗死体积明显减小,P-L组脑梗死体积较IPOC组进一步缩小(P<0.05)。SO组的PI3K mRNA及其蛋白的表达量较少,MCAO组的PI3K表达明显增高(P<0.01);IPOC组、P-L组的PI3K表达水平较MCAO组升高(P<0.01);P-L组PI3K表达较IPOC组升高(P<0.05)。结论脑缺血损伤;脑缺血后处理具有神经保护作用,其机制可能与其诱导PI3K的表达有关,水蛭注液可进一步促进其表达而发挥神经保护作用。

引用

页码：158 / 161

页数：4

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