充血性心力衰竭病人心肌细胞凋亡及Fas/FasL系统的调节作用

被引：7

作者：

张顺业

孙宗全

机构：

[1] 华中科技大学同济医学院协和医院心脏外科

来源：

山西医科大学学报 | 2001年 / 06期

关键词：

心力衰竭,充血性; 心肌; 脱噬作用; caspase-3; Fas; FasL; 氧化性应激;

D O I：

暂无

中图分类号：

R541.61 [];

学科分类号：

摘要：

目的　观察充血性心力衰竭 (CHD)病人心肌细胞是否存在细胞凋亡以及Fas/FasL蛋白、caspase 3和心肌氧化性应激的调节作用。方法　实验以进行常规CPB的无肺动脉高压的ASD病人为对照组 (n =5 ) ,以合并CHD的RHD病人为观察组(n =5 )。体外循环术前留取右心室标本。用原位TUNEL技术检测凋亡心肌细胞。用流式细胞仪技术测定右心室Fas和FasL蛋白表达。用Z DEVD AMC底物降解法和TBARS方法分别测定右心室心肌caspase 3酶活性和脂质过氧化物含量(MDA)。结果　RHD病人右心室心肌细胞凋亡数、caspase 3活性、MDA以及Fas、FasL蛋白表达均明显高于ASD病人 (P分别为 0 0 2 6 ,0 0 13,0 0 0 2 ,0 0 0 4和 0 0 33)。结论　心肌细胞凋亡参与CHD的病理生理过程。CHD病人的心肌细胞凋亡与心肌细胞Fas、FasL蛋白表达上调、caspase 3激活以及心肌氧化性应激增高有关

引用

页码：492 / 493

页数：2

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