莱克多巴胺单克隆抗体的制备与初步鉴定

被引:5
作者
曲勍
齐孟文
机构
[1] 中国农业大学核技术农业应用实验室
关键词
莱克多巴胺; β-肾上腺素激动剂; 单克隆抗体;
D O I
暂无
中图分类号
S852.43 [];
学科分类号
摘要
为了对莱克多巴胺进行酶联免疫法测定,经由混合酸酐法合成莱克多巴胺免疫原,免疫BALB/c小鼠,通过杂交瘤单克隆化技术最终得到了1株名为3E1-C9-E10的能稳定分泌抗莱克多巴胺的单克隆抗体的杂交瘤细胞株。经ELISA测定,细胞培养上清的效价为1×105,腹水的效价达1×107以上,与多巴酚丁胺有24%的交叉反应率,与沙丁胺醇、克伦特罗没有交叉反应率。
引用
收藏
页码:393 / 396
页数:4
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