用UDPE技术检测和鉴定鼠疫耶尔森氏菌

被引:12
作者
郭兆彪
张敏丽
汪晓辉
王津
杨瑞馥
张春华
丛显斌
张景林
机构
[1] 军事医学科学院微生物流行病研究所,军事医学科学院微生物流行病研究所,军事医学科学院微生物流行病研究所,军事医学科学院微生物流行病研究所,军事医学科学院微生物流行病研究所,全国鼠疫布氏菌病防治基地,全国鼠疫布氏菌病防治基地,全国鼠疫布氏菌病防治基地
关键词
尿嘧啶糖基化酶; PCR; 鼠疫耶尔森尔菌; 微孔板杂交; UDPE;
D O I
暂无
中图分类号
R446.5 [微生物学检验];
学科分类号
100208 ;
摘要
我们建立了用UDG(尿嘧啶糖基化酶)防PCR产物污染,以两个不同基因为靶序列扩增检测同一病原菌和用微孔板杂交-酶联显色检测PCR产物的UDPE(UDG-Duplex PCR-EIA)技术,并用于鼠疫耶尔森氏菌的检测与鉴定。结果表明,该系统可以特异地检测和鉴定鼠疫耶尔森菌,检测灵敏度可以达到1CFU/ml。
引用
收藏
页码:262 / 265+321 +321
页数:5
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