16S nested-PCR技术检测玉米细菌性枯萎病菌(英文)

被引:9
作者
吴琼
陈枝楠
范怀忠
金显忠
机构
[1] 深圳出入境检验检疫局
[2] 华南农业大学资环学院植物病理学系
关键词
玉米细菌性枯萎病菌; 16SrDNA; 检测; 巢式PCR;
D O I
10.13926/j.cnki.apps.2005.05.006
中图分类号
S435.131.49 [];
学科分类号
摘要
玉米细菌性枯萎病是玉米上的重要种传病害,病原菌为Pantoea stewartiisubsp.stewartii。本研究设计了16S通用引物,扩增该病菌及其近似种的16S rDNA,通过序列测定和分析,针对该病菌设计了特异性引物,采用nested-PCR技术,能够准确地区别该病菌及其近似种,检测的灵敏度在DNA水平上达到10-3pg级,检测活菌则达到2 cfu。检测人工污染的玉米种子时,不受种子提取液中其它物质的干扰,灵敏度依然达到2 cfu。
引用
收藏
页码:420 / 427
页数:8
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