导入LPAAT和KCS基因对油菜种子芥酸含量的影响

被引:13
作者
陈柳 [1 ]
毛善婧 [1 ]
陆莉 [1 ]
储成才 [2 ]
SONNTAG Karin [3 ]
王幼平 [1 ]
机构
[1] 扬州大学生物科学与技术学院
[2] 中国科学院遗传与发育生物学研究所
[3] Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops,Groβ Luesewitz
关键词
甘蓝型油菜; 溶血性磷脂酸酰基转移酶; 酮脂酰辅酶A合成酶; 芥酸;
D O I
暂无
中图分类号
S565.4 [油菜籽(芸薹)];
学科分类号
摘要
采用农杆菌介导法,以下胚轴为转化受体,将溶血性磷脂酸酰基转移酶(LPAAT)和酮脂酰辅酶A合成酶(KCS)基因导入2种不同基因型(“Drakkar”和“Maplus”)的甘蓝型油菜,共获得247株再生植株,其再生频率分别为15.1%和2.0%。PCR检测和Southern杂交分析证实,外源基因已整合到油菜基因组中,转化频率分别为3.6%和0.7%。种子中芥酸含量因外源基因的导入使零芥酸油菜“Drakkar”提高到10.5%,高芥酸品种“Maplus”提高了5.0%,最高达62.8%。
引用
收藏
页码:1174 / 1178
页数:5
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