喜树碱诱导人白血病HL-60细胞凋亡过程中端粒酶的调控(英文)

被引：5

作者：

蒋建飞

刘卫军

丁健

机构：

[1] 中国科学院上海药物研究所,中国科学院上海药物研究所,中国科学院上海药物研究所上海,中国,上海,中国,上海,中国

来源：

Acta Pharmacologica Sinica | 2000年 / 08期

关键词：

喜树碱; 细胞凋亡; 端粒酶; bcl-2基因;

D O I：

暂无

中图分类号：

R733.7 [白血病];

学科分类号：

100214 ;

摘要：

目的:研究在喜树碱诱导人白血病HL—60细胞凋亡过程中端粒酶的调节变化规律.方法:用MTT法测定药物对细胞存活率的影响;用琼脂糖电泳及流式细胞术检测和定量凋亡的发生;用以PCR为基础的TRAP法测定端粒酶活力;逆转录PCR检测凋亡过程中bcl-2及端粒酶亚基hTR、hEST2/hTERT和TLPl/TPl的基因表达水平的变化.结果:端粒酶活力伴随喜树碱诱导HL-60细胞凋亡的发生而逐渐降低,在此过程中端粒酶各亚基的mRNA水平无可见性变化,而bcl-2的基因表达水平则相应下调.结论:端粒酶活力的下调和喜树碱诱导的HL-60凋亡密切相关,端粒酶活力的阻断并非发生在其亚基基因转录水平,bcl-2对端粒酶活力的调节也不是通过影响端粒酶亚基的转录水平来实现的.

引用

页码：90 / 95

页数：6

共 18 条

[1]

Regulation of telomerase activity in immortal cell lines. Ho SE,Wright WE,Shay JW. Molecular and Cellular Biology . 1996

[2]

A mammalian telomerase-associated protein. Harrington L,McPhail T,Mar V,Zhou W,Oulton R,Bass MB,et al. Science . 1997

[3]

Advances in quantification and characterization of telomerase activity by the telomeric repeat amplification protocol(TRAP). Kim NW,Wu F. Nucleic Acids Research . 1997

[4]

Leukemia[P]. 英国专利:GB0221802D0,2002-10-30

[5]

Feasibilityty of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Alley MC,Scudiero DA,Monks A,Hursey ML,Czerwinski MJ,Fine DL,et al. Cancer Research . 1988

[6]

Apoptosis and disease. Carson DA,Ribeiro JM. The Lancet . 1993

[7]

Regulation of protein phosphatase 2A activity by caspase-3 during apoptosis. Santoro MF,Annand RR,Robertson MM,Peng YW,Brady MJ. Journal of Biological Chemistry . 1998

[8]

Anti-apoptotic role of telomerase in pheochromocytoma cells. Fu WM,Begley JG,Killen MW,Mattson MP. Journal of Biological Chemistry . 1999

[9]

TLP1: a gene encoding a protein component of mammalian telomerase is a novel member of WD repeat family. Nakayama J,Saito M,Nakamura H,Matsuura A,Ishikawa F. Cell . 1997

[10]

Protein phosphatase 2A inhibits nuclear telomerase activity in human breast cancer cells. Li H,Zhao LL,Funder JW,Liu JP. Journal of Biological Chemistry . 1997

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