PCR检测乳品中金黄色葡萄球菌

被引：37

作者：

杨洋 ^{[1
]}

张伟 ^{[1
]}

袁耀武 ^{[2
]}

钟晓英 ^{[3
]}

马雯 ^{[1
]}

机构：

[1] 河北农业大学食品科技学院微生物检测研究室

[2] 河北农业大学食品科技学院微生物检测研究

[3] 河北省保定市疾病预防控制中心

来源：

中国农业科学 | 2006年 / 05期

关键词：

PCR; 检测; 乳品; 金黄色葡萄球菌;

D O I：

暂无

中图分类号：

TS252 [乳品加工工业];

学科分类号：

083203 ;

摘要：

目的利用PCR技术,无需增菌,直接检测乳品中的金黄色葡萄球菌。方法通过溶剂提取的方法从人工样品中提取模板,以金黄色葡萄球菌耐热核酸酶基因(nuc)为靶基因,经过PCR扩增得到279bp的产物。经过DNA测序证实该产物为目的扩增产物。采用PCR方法实际检测了乳品中的金黄色葡萄球菌,同时,与国标GB4789.10—94方法及两种快速检测致病性金黄色葡萄球菌测试片PetrifilmRSA.CountPlate及Baird-parker+RPFAgar进行了比较。结果PCR方法的灵敏度高,全脂乳和脱脂乳检测的检出限为10CFU/ml,奶酪检测的检出限为55CFU/g,可在6h内完成对乳品中金黄色葡萄球菌的检测,比目前普遍采用的先增菌再进行PCR检测的方法缩短了12～24h。与国标方法相比,PCR方法的符合率为94.3%,敏感性为100%。结论采用溶剂提取制备模板的方法可有效的用于PCR直接检测(无需增菌)乳及乳制品中的金黄色葡萄球菌。

引用

页码：990 / 996

页数：7

共 11 条

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