水稻胚胎发育过程相关基因的鉴定(简报)(英文)

被引：2

作者：

袁静

任育军

赵洁

机构：

[1] 武汉大学生命科学学院植物发育生物学教育部重点实验室

来源：

分子细胞生物学报 | 2008年 / 03期

关键词：

胚胎发育; 抑制差减杂交; 基因表达; 水稻;

D O I：

暂无

中图分类号：

S511 [稻]; Q943 [植物细胞遗传学];

学科分类号：

0901 ; 071007 ; 090102 ;

摘要：

为了研究水稻胚胎发育的分子机制,我们运用抑制差减杂交(SSH)技术鉴定了胚胎发育早期(授粉后5-7天)和晚期(授粉后15-17天)优势表达的基因。结果发现在胚胎发育早期和晚期优势表达的表达序列标签(EST)分别为47个和15个,这些EST可分为新陈代谢、蛋白合成、蛋白修饰、细胞防御或胁迫、转运运输、转译、DNA或RNA结合等多种类别,其中有32%的EST在GenBank的生物信息数据库中没有同源序列。从两个SSH文库中随机抽取11个EST分别在5DAP和15DAP水稻分化的胚胎中进行RT-PCR验证,结果显示SSH文库所获得的EST符合建库要求。对这些EST所代表的基因作进一步研究将有助于了解其在水稻胚胎发育中的生物学功能。

引用

页码：238 / 244

页数：7

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