日本血吸虫成虫31/32KD抗原的纯化及其免疫化学特性

被引:2
作者
汪世平
易新元
曾宪芳
机构
[1] 湖南医科大学寄生虫学教研室,湖南医科大学寄生虫学教研室,湖南医科大学寄生虫学教研室研究生,导师,导师
关键词
日本血吸虫; 抗原; 虫卵可溶性抗原~*; 成虫31/32KD抗原~*; 分离与提纯; 免疫化学;
D O I
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中图分类号
学科分类号
摘要
本文采用超凝胶柱层析法分离纯化日本血吸虫成虫31/32KD抗原。分离的抗原经银染色及免疫印迹法分析证明已达免疫纯及生化纯级。该抗原PAS染色呈阴性,经过碘酸钠处理后不失去活性,在琼脂糖凝胶电泳中趋向阳极。鉴于血吸虫成虫31/32KD组分是一种主要血清学抗原,它的分离纯化为改进和标化血吸虫病的诊断提供了条件。
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页码:307 / 311
页数:5
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