大鼠牙本质基质蛋白1单克隆抗体的制备、鉴定及其在牙胚发育过程中的表达

被引：2

作者：

高杰

吴补领

汪平

机构：

[1] 第四军医大学口腔医学院,第四军医大学口腔医学院,第四军医大学口腔医学院陕西西安,陕西西安,陕西西安

来源：

牙体牙髓牙周病学杂志 | 2002年 / 04期

基金：

高等学校骨干教师资助计划;

关键词：

牙本质基质蛋白1; 细胞融合; 杂交瘤; 单克隆抗体; 免疫组化;

D O I：

10.15956/j.cnki.chin.j.conserv.dent.2002.04.006

中图分类号：

R780.2 [口腔病理学];

学科分类号：

100301 ;

摘要：

目的 :制备抗大鼠牙本质基质蛋白 1(dentinmatrixprotein 1,DMP1)的单克隆抗体 ,研究其在牙胚发育过程中的时空表达。方法 :以重组的DMP1为抗原 ,免疫Balb/c小鼠 ,通过细胞融合 ,建立分泌单克隆抗体的杂交瘤细胞系。用免疫组化方法检测DMP1在牙胚发育过程中的表达。结果 :获得了 2株稳定分泌抗DMP1特异性抗体的杂交瘤细胞 ,两株单克隆抗体的亚类均为IgG1,与重组的DMP1蛋白和牙齿组织总蛋白均可特异性反应。DMP1在磨牙和切牙分泌性成牙本质细胞胞浆顶端及切牙成釉细胞中有表达。结论 :通过杂交瘤技术 ,获得了 2株抗DMP1的单克隆抗体。DMP1在大鼠磨牙发育过程中的分布具有时空特异性 ,可能促进牙本质矿化 ,诱导前成釉细胞向成釉细胞分化

引用

页码：178 / 181+230 +230

页数：5

共 9 条

[1]

Gene expression patterns of murine dentin matrix protein 1 ( DMP1 ) and dentin sialophosphoprotein(DSPP) suggest distinct developmental functions in vivo. D’Souza RN,cavender A,MacDougall M,et al. Journal of Bone and Mineral Research . 1997

[2]

Recombinant expression and characterization of dentin matrix protein 1. Srinivasan R,Chen B,GeorgeA,et al. Connective Tissue Research . 1999

[3]

Identification of a novel isoform of mouse dentin matrix protein 1: spatial expression in mineralized tissues. MacDougall M,Gu TT,Luan X,et al. Journal of Bone and Mineral Research . 1998

[4]

Inositol hexasulphate, a casein kinase inhibitor, alters the distribution of dentin matrix protin 1 in cultured embryonic mouse tooth germs. Septier D,Torres-Quintana MA,Menashi S,et al. European Journal of Oral Sciences . 2001

[5]

A comparative study of sialic acid-rich proteins in rat bone and dentin. Qin C,Brunn JC,George A,et al. European Journal of Oral Sciences . 2001

[6]

Characterization of a novel dentin matrix phosphoprotein. George A,Sabasy B,Simonian PAL,et al. Journal of Biological Chemistry . 1993

[7]

Measurement of monoclonal antibody affinity by non - competitive enzyme immunoassay. Beatty JD,Barbara G,William G,et al. Journal of Immunological Methods . 1987

[8]

Development of a monoclonal antibody against dentin phosphophoryn: a tool to study odontoblastic activity. Laboux O,Menanteau J,Pelhate S,et al. Hybridoma . 1994

[9]

In situ localization and chromosomal mapping of the AG1 ( Dmp1 ) gene. George A,Gui J,Jenkins NA,et al. Journal of Histochemistry and Cytochemistry . 1994

← 1 →