尖锐湿疣病变的人乳头瘤病毒6型L1序列多态性分析

被引：7

作者：

洪少林

王家璧

李平川

周玲

司静懿

许雪梅

郭秀婵

曾毅

机构：

[1] 中国医学科学院中国协和医科大学北京协和医院,中国医学科学院中国协和医科大学北京协和医院,中国疾病预防控制中心病毒病预防控制所,中国疾病预防控制中心病毒病预防控制所,中国医学科学院中国协和医科大学基础医学研究所,中国医学科学院中国协和医科大学基础医学研究所,中国

来源：

病毒学报 | 2002年 / 02期

关键词：

人乳头瘤病毒6型; 聚合酶链式反应; L1开放读码框; 基因序列多态性;

D O I：

10.13242/j.cnki.bingduxuebao.001386

中图分类号：

R752.53 [];

学科分类号：

摘要：

采用PCR方法从协和医院尖锐湿疣患者皮损中检测人乳头瘤病毒 (HPV) ,并通过限制性片断长度多态性分析分型发现 ,HPV6型为主要感染型别 ,其次是HPV11型。根据临床特征选择主要致病型HPV6 8个分离株 ,扩增L1晚期基因 ,构建重组测序质粒 ,双脱氧法测序并分析L1区的核苷酸和氨基酸序列变异状况。结果表明 ,HPV6L1基因序列发生碱基替换的区域主要有四个区 ,包括SR1(5 911～ 6 10 4 ) ,SR2 (6 2 17～ 6 2 73) ,SR3(6 5 4 0～6 6 6 1)和SR4 (70 6 2～ 72 5 0 )。少数的碱基替换导致错义突变 ,推导的蛋白质一级结构中有 0～ 3个氨基酸发生变异 ,但抗原性强弱与原型HPV6基本一致

引用

页码：102 / 107

页数：6

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