基于网络药理学对白术治疗阿尔茨海默症的机制探讨

被引：14

作者：

胡倩

刘育铖

毛思宇

冯星

机构：

[1] 湖南师范大学医学院

[2] 湖南师范大学小分子靶向药物研究与创制湖南省重点实验室

来源：

中南药学 | 2020年 / 18卷 / 03期

基金：

湖南省自然科学基金;

关键词：

网络药理学; 白术; 阿尔茨海默症; 关键靶标; 双白术内酯;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

100806 [中药药理学];

摘要：

目的利用网络药理学探讨中药白术"成分-靶标-疾病"的相互关系及其有效成分治疗阿尔茨海默症的作用机制。方法从中药系统药理学数据库(TCMSP)中筛选白术活性成分,利用Cytoscape 3.2.1软件将活性成分、成分靶标及相关疾病构建可视化网络图,通过生物信息学方法进行关键靶标的GO功能分析和KEGG信号通路富集分析;并通过MTT细胞增殖实验与活性氧(ROS)荧光探针实验验证白术有效成分对损伤神经细胞的保护作用。结果通过网络药理学方法收集到白术共55个化学成分,进一步筛选出18个活性成分,其中包括主要有效成分双白术内酯。网络富集得到17个关键靶标,72种相关疾病及5条通路。这些成分靶标,疾病靶标和信号通路均表明白术可以有效作用于阿尔茨海默症。细胞实验表明白术的有效成分双白术内酯能显著提高损伤神经细胞的细胞活力,并减少活性氧的生成。结论本研究筛选了白术的有效成分、关键靶标及相关通路,为其治疗阿尔茨海默症的作用机制研究提供了科学依据。

引用

页码：427 / 434

页数：8

共 25 条

[1]

Biatractylolide Modulates PI3K-Akt-GSK3 β -Dependent Pathways to Protect against Glutamate-Induced Cell Damage in PC12 and SH-SY5Y Cells.[J].Li Zhu;Ning Ning;Yu Li;Qiu-Fang Zhang;Yong-Chao Xie;Maida Irshad;Xing Feng;Xiao-Jun Tao;Juntra Karbwang.Evidence-Based Complementary and Alternative Medi.2017,

[2]

Primary Investigation for the Mechanism of Biatractylolide from Atractylodis Macrocephalae Rhizoma as an Acetylcholinesterase Inhibitor.[J].Yong-Chao Xie;Ning Ning;Li Zhu;Dan-Ning Li;Xing Feng;Xiao-Ping Yang;Hyunsu Bae.Evidence-Based Complementary and Alternative Medi.2016,

[3]

Network pharmacology: A new approach to unveiling Traditional Chinese Medicine.[J].Xiao-Ming WU;Chun-Fu WU.Chinese Journal of Natural Medicines.2015, 1

[4]

TCMSP: a database of systems pharmacology for drug discovery from herbal medicines..[J].Jinlong Ru;Peng Li;Jinan Wang;Wei Zhou;Bohui Li;Chao Huang;Pidong Li;Zihu Guo;Weiyang Tao;Yinfeng Yang;Xue Xu;Yan Li;Yonghua Wang;Ling Yang.J. Cheminformatics.2014, 1

[5]

Network Pharmacology: A Rosetta Stone for Traditional Chinese Medicine [J].

Hao, Da Cheng ;

Xiao, Pei Gen .

DRUG DEVELOPMENT RESEARCH, 2014, 75 (05) :299-312

[6]

An Integrative Platform of TCM Network Pharmacology and Its Application on a Herbal Formula; Qing-Luo-Yin.[J].Bo Zhang;Xu Wang;Shao Li;Aiping Lu.Evidence-Based Complementary and Alternative Medicine.2013,

[7]

Systems Pharmacology: Network Analysis to Identify Multiscale Mechanisms of Drug Action.[J].Shan Zhao;Ravi Iyengar.Annual Review of Pharmacology and Toxicology.2012, 1

[8]

Potential Role of Metabolomic Approaches for Chinese Medicine Syndromes and Herbal Medicine [J].

Sun, Hui ;

Zhang, Aihua ;

Wang, Xijun .

PHYTOTHERAPY RESEARCH, 2012, 26 (10) :1466-1471

[9]

LOSS OF NONPHOSPHORYLATED NEUROFILAMENT IMMUNOREACTIVITY IN TEMPORAL CORTICAL AREAS IN ALZHEIMER'S DISEASE [J].

Thangavel, R. ;

Sahu, S. K. ;

Van Hoesen, G. W. ;

Zaheer, A. .

NEUROSCIENCE, 2009, 160 (02) :427-433

[10]

Multi-target therapeutics: when the whole is greater than the sum of the parts.[J].Grant R. Zimmermann;Joseph Lehár;Curtis T. Keith.Drug Discovery Today.2006, 1

← 1 2 3 →