柔红霉素在HL-60ADR细胞内的异常分布

被引:9
作者
龚玉萍
王燕婷
陈芳源
韩洁英
邵念贤
方智雯
欧阳仁荣
机构
[1] 上海第二医科大学附属仁济医院血液内科,上海第二医科大学附属仁济医院血液内科,上海第二医科大学附属仁济医院血液内科,上海第二医科大学附属仁济医院血液内科,上海第二医科大学附属仁济医院血液内科,上海第二医科大学附属仁济医院血液内科,上海第二医科大学附属仁济医院血液
关键词
抗药性; 多药; 共聚焦激光扫描显微镜; 细胞株; HL60ADR; 药物分布;
D O I
暂无
中图分类号
R979.1 [抗肿瘤、抗癌药物];
学科分类号
摘要
目的 了解化疗药物在非Pgp耐药细胞株HL 6 0 ADR细胞内的异常分布与耐药的关系。方法 采用共聚焦激光扫描显微镜观察、荧光染色、MTT、逆转录 聚合酶链反应 (RT PCR)等方法研究柔红霉素 (DNR)在HL 6 0 ADR细胞内的分布及与耐药的关系 ,以及维拉帕米、布雷菲尔得菌素、氯喹对细胞内DNR异常分布的影响。结果 与DNR在HL 6 0细胞核、胞浆均匀分布不同 ,在HL 6 0 ADR ,DNR呈点棘状分布于细胞浆外周区 ,核内DNR荧光很少 ;维拉帕米、布雷菲尔得菌素可逆转DNR在HL 6 0 ADR的异常分布 ,而氯喹无此作用。结论 DNR在耐药细胞的异常分布与肿瘤细胞耐药的形成有关。
引用
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页码:28 / 30+57
页数:4
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