糖皮质激素对口腔扁平苔藓辅助性T细胞平衡的影响

被引：22

作者：

周刚

樊明文

刘君炎

机构：

[1] 武汉大学口腔医学院黏膜科,武汉大学口腔医学院口腔生物医学工程教育部重点实验室,武汉大学医学院微生物学和免疫学研究室,

来源：

中华口腔医学杂志 | 2005年 / 02期

关键词：

扁平苔藓,口腔; 地塞米松; Th1细胞; Th2细胞;

D O I：

暂无

中图分类号：

R781.5 [口腔粘膜病];

学科分类号：

100302 ;

摘要：

目的　明确口腔扁平苔藓 (OLP)患者的Th1 /Th2免疫应答模式,探讨糖皮质激素对OLP患者辅助性T细胞平衡的影响。方法　密度梯度离心法分离OLP患者和对照组外周血单个核细胞,分别用植物血凝素(PHA)和地塞米松刺激OLP患者外周血单个核细胞,采用酶联免疫吸附法,检测培养上清液中干扰素γ(IFN γ)和白细胞介素 4(IL 4)的含量;应用半定量逆转录聚合酶链反应技术,检测培养细胞中IFN γ和IL 4mRNA的表达水平。结果　外周血单个核细胞经PHA诱导培养,OLP患者组IFN γ的水平低于健康对照组 (P<0 05),IL 4的水平高于对照组,但差异无统计学意义(P>0 05),IFN γ/IL 4的比值低于对照组 (P<0 05 )。地塞米松可抑制IFN γ和IL 4的水平(P<0 01),且对IFN γ的抑制作用较IL 4更为显著。结论　OLP患者存在Th1 /Th2的平衡失调,为Th2占优势的免疫应答,糖皮质激素对OLP的Th1 /Th2细胞因子均有抑制作用。

引用

页码：14 / 17

页数：4

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