HO-1/CO系统抑制AngⅡ诱导的大鼠心肌细胞凋亡

被引：4

作者：

谷颖

王爱红

陈东昌

鹿克风

机构：

[1] 山东大学附属省立医院心内科

来源：

基础医学与临床 | 2013年 / 33卷 / 09期

关键词：

血红素氧合酶-1; 内源性一氧化碳; 心肌细胞; 凋亡;

D O I：

10.16352/j.issn.1001-6325.2013.09.013

中图分类号：

R541.6 [血液循环衰竭];

学科分类号：

100201 [内科学];

摘要：

目的探讨血红素氧合酶-1/一氧化碳(HO-1/CO)系统对血管紧张素Ⅱ(AngⅡ)诱导的大鼠心肌细胞凋亡的影响。方法原代培养新生Wistar大鼠心肌细胞,随机分为:对照组、AngⅡ组、AngⅡ+氯化血红素(hemin)(HO-1诱导剂)组和AngⅡ+锌原卟啉-9(ZnppIX)(HO-1抑制剂)组。用real-time PCR及Western blot检测心肌细胞HO-1mRNA和蛋白的表达,比色法测定细胞培养上清液中碳氧血红蛋白(COHb)含量,流式细胞仪检测细胞凋亡。结果AngⅡ组心肌细胞HO-1 mRNA、蛋白、COHb含量和细胞凋亡均明显高于对照组(P<0.05),AngⅡ+hemin组HO-1mRNA、蛋白、COHb含量进一步升高(P<0.05),而细胞凋亡回降(P<0.05),但仍高于对照组(P<0.05),AngⅡ+ZnPPIX组仅细胞凋亡显著升高(P<0.05),其他指标无显著变化。结论 HO-1/CO系统对AngⅡ诱导的心肌细胞凋亡具有抑制作用。

引用

页码：1150 / 1154

页数：5

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