神经肽在去卵巢大鼠骨组织中表达差异的实验研究

被引：5

作者：

王双磊 ^{[1
]}

韩燚 ^{[1
]}

谢玮鑫 ^{[1
]}

李展春 ^{[1
]}

肖洁 ^{[2
]}

机构：

[1] 上海交通大学医学院附属仁济医院骨关节外科

[2] 上海交通大学医学院附属仁济医院麻醉科

来源：

中国骨质疏松杂志 | 2018年 / 24卷 / 04期

关键词：

神经肽; 去卵巢; 假手术; 骨质疏松;

D O I：

暂无

中图分类号：

R580 [];

学科分类号：

摘要：

目的探讨神经肽P物质(substance P,SP),降钙素基因相关肽(calcitonin gene-related peptide,CGRP),血管活性肠肽(vasoactive intestinal peptide,VIP)和神经肽Y(neuropeptide Y,NPY)在去卵巢组(ovariectomized,OVX)大鼠和假手术组(sham-operated,Sham)大鼠胫骨干骺端表达的差异以及与骨密度(bone mineral density,BMD)的相关性。方法 12只6月龄雌性未孕SD大鼠随机分为Sham组和OVX组,每组6只。造模后12 w两组大鼠称量体重后处死,取大鼠子宫称重,双能X线检测大鼠胫骨干骺端BMD,免疫组织化学法观察两组大鼠右侧胫骨干骺端SP,CGRP,VIP和NPY表达,取平均光密度(mean optical density,MOD)值,分析其与BMD之间相关性。结果造模后12 w,与Sham组相比较,OVX组大鼠体重增加,子宫湿重和BMD值降低,免疫组织化学法观察发现SP,CGRP和VIP的MOD值降低,NPY的MOD值升高,差异具有统计学意义(P<0.01)。大鼠SP,VIP的MOD值与BMD值成正相关关系(r=0.746,P=0.005;r=0.591,P=0.043),NPY的MOD值与BMD值成负相关关系(r=-0.666,P=0.018)。结论骨质疏松可能与骨组织中神经肽的表达量改变有关,但其中具体机制有待进一步研究。

引用

页码：437 / 440+457 +457

页数：5

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