呋喃它酮代谢物人工抗原及多克隆抗体的制备

被引：2

作者：

刘迎春

蒋蔚

陈永军

高艺林

石金磊

王权

机构：

[1] 中国农业科学院上海兽医研究所兽医公共卫生实验室农业部动物寄生虫学重点实验室

来源：

畜牧与兽医 | 2013年 / 45卷 / 02期

关键词：

呋喃它酮代谢物; 人工抗原; 化学合成; 鉴定;

D O I：

暂无

中图分类号：

S859.84 [动物性食品中化学物残留];

学科分类号：

0906 ;

摘要：

本文利用对醛基苯甲酸(CPA)与呋喃它酮代谢物(AMOZ)进行衍生化,得到衍生物CPAMOZ,并通过质谱法对其结构进行确证。然后采用混合酸酐法将CPAMOZ与载体蛋白偶联,采用紫外扫描(UV)法对偶联物进行验证。用偶联成功的人工全抗原(CPAMOZ-BSA)免疫BALB/c小鼠,间接ELISA测定小鼠血清抗体效价为1∶8×104,对NPAMOZ的半数抑制量为26.55μg/L,间接竞争ELISA鉴定小鼠血清多克隆抗体,与NPAMOZ、CPAMOZ的交叉反应率分别为100%、44.8%,与AMOZ、对醛基苯甲酸、对硝基苯甲醛以及同类其他三种药物呋喃妥因、呋喃西林、呋喃唑酮等原药及其代谢物以及代谢物衍生物的交叉反应均小于0.01%。结果表明,制备的AMOZ多克隆抗体效价高、特异性强,灵敏度高,为进一步制备AMOZ单克隆抗体和研制快速筛选检测试剂盒奠定了基础。

引用

页码：69 / 73

页数：5

共 6 条

[1]

Development of a monoclonal antibody-based enzyme-linked immunosorbent assay for detection of the furaltadone metabolite, AMOZ, in fortified shrimp samples[J] . Umaporn Pimpitak,Songchan Putong,Kittinan Komolpis,Amorn Petsom,Tanapat Palaga. Food Chemistry . 2009 (3)

[2]

Determination of nitrofuran metabolites in milk by liquid chromatography–electrospray ionization tandem mass spectrometry[J] . Lech Rodziewicz. Journal of Chromatography B . 2008 (1)

[3]

Simultaneous determination of 5-nitroimidazoles and nitrofurans in pork by high-performance liquid chromatography–tandem mass spectrometry[J] . Xi Xia,Xiaowei Li,Suxia Zhang,Shuangyang Ding,Haiyang Jiang,Jiancheng Li,Jianzhong Shen. Journal of Chromatography A . 2008 (1)

[4]

Multi-residue monitoring for the simultaneous determination of five nitrofurans (furazolidone, furaltadone, nitrofurazone, nitrofurantoine, nifursol) in poultry muscle tissue through the detection of their five major metabolites (AOZ, AMOZ, SEM, AHD, DNSAH) by liquid chromatography coupled to electrospray tandem mass spectrometry—In-house validation in line with Commission Decision 657/2002/EC[J] . Eric Verdon,Pierrick Couedor,Pascal Sanders. Analytica Chimica Acta . 2007 (1-2)

[5]

Analysis of nitrofuran metabolic residues in salt by liquid chromatography–tandem mass spectrometry[J] . A.S Pereira,L.C Pampana,J.L Donato,G De Nucci. Analytica Chimica Acta . 2004 (1)

[6]

Pharmacological, Therapeutic and Toxicological Properties of Furazolidone: Some Recent Research[J] . B.H. Ali. Veterinary Research Communications . 1999 (6)

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