环境生物修复中高效基因工程菌的构建策略

被引:20
作者
刘和
陈英旭
机构
[1] 浙江大学环境工程系
[2] 浙江大学环境工程系 浙江杭州
[3] 浙江杭州
关键词
生物修复; 基因工程菌; 污染物降解基因; 环境生物技术;
D O I
暂无
中图分类号
X172 [环境微生物学];
学科分类号
071012 ; 0713 ;
摘要
通过对环境污染物的生物降解机制及妨碍污染物降解的因素进行分析 ,提出了几种高效基因工程降解菌的构建策略 ,包括重组污染物降解基因以优化污染物降解途径、重组污染物摄入相关基因以改善对污染物的生物可利用性和重组环境不利因子抵抗基因以增强其环境适应性等
引用
收藏
页码:92 / 96
页数:5
相关论文
共 9 条
[1]  
Bioremediation: towards a credible technology. Ian M head. Microbiology . 1998
[2]  
Rapid evolution of a protein in vitro by DNA shuffing. Stemmer W P C. Nature . 1994
[3]  
a catabolic plasmid-encoded receptor required for chmotaxis of pseudomonas putida to the aromatic hydrocarbon naphthalene. Grimm A C,Harwood C S,NahY. Journal of Bacteriology . 1999
[4]  
Engineering bacteria for bioremediation. Pieper D H,Reineke W. Current Opinion in Biotechnology . 2000
[5]  
Environmental biotechnology towards sustainability. Lawrence P W,Neil C B. Current Opinion in Biotechnology . 2000
[6]  
Engineering a recombinant Deinoccus Radiofurans for organopollutant degradation in radioactive mixed waste environments. Lange C C,Wackett L P,Minton K W,et al. Nature Biotechnology . 1998
[7]  
Engineering Deinoccus Radiofurans for metal remediation in radioactive mixed waste environments. Brim H,McFarlan S C,Fredrickson J K,et al. Nature Biotechnology . 2000
[8]  
Engineering dioxygenase for efficient degradation of environmental pollutants. Furukawa K. Current Opinion in Biotechnology . 2000
[9]  
Designing recombinant Pseudomonas strains to enhance biodesulfurization. Gallardo M E,Ferrandez A,de Lorenzo V,et al. Journal of Bacteriology . 1997