大鼠肢体缺血再灌注致肺损伤时肺组织中血红素氧合酶的表达

被引：7

作者：

周君琳

凌亦凌

王俊霞

张君岚

史中立

丛斌

岳凤鸣

丁春华

机构：

[1] 河北医科大学病理生理教研室!河北石家庄,河北医科大学病理生理教研室!河北石家庄,河北医科大学分子生物学研究中心!河北石家庄,河北医科大学病理生理教研室!河北石家庄,河北医科大学病理生理教研室!河北石家庄,河北医科大学分子生物学研究中心!

来源：

中国病理生理杂志 | 2001年 / 05期

关键词：

血红素氧合酶; 再灌注损伤; 肺; 四肢;

D O I：

暂无

中图分类号：

R363 [病理生理学];

学科分类号：

100104 ;

摘要：

目的 :观察大鼠肢体缺血再灌注致肺损伤时肺组织中HO - 1表达的变化。方法 :采用夹闭大鼠腹主动脉下段造成双下肢缺血和再灌注性肺损伤模型 ,分别采集假手术组 ,缺血 4h组及缺血 4h再灌注 4、8、16、2 4、48h组肺组织标本 ,以Northern印迹、Western印迹及免疫组化分析 ,观察HO - 1表达的变化。结果 :假手术组和单纯缺血组未见HO - 1mRNA表达 ;再灌注 4h可见其表达信号 ,且随着再灌注时间延长表达信号逐渐增强 ,到 16h时达到高峰 ,之后渐减弱 ,48h时已消失。蛋白表达水平与mRNA表达一致。免疫组化研究显示 ,HO - 1阳性信号主要出现在气道上皮细胞、血管平滑肌细胞和肺泡巨噬细胞 ,而假手术组和单纯缺血组均未见阳性信号。结论 :假手术及肢体的单纯缺血未诱发肺组织HO - 1的表达 ,而肢体缺血再灌注可诱发其表达 ,并具有时相变化

引用

页码：55 / 57+99

页数：4

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