蒺藜总皂苷灌胃大鼠体内海柯皂苷元的代谢与分布

被引:5
作者
陈原国
瞿伟菁
杨乃乙
蒋子威
机构
[1] 华东师范大学生命科学学院
关键词
蒺藜总皂苷; 海柯皂苷元; RP-HPLC-UV; LC-UV-MS; 大鼠; 代谢分布;
D O I
10.16333/j.1001-6880.2006.06.009
中图分类号
R285 [中药药理学];
学科分类号
1008 ;
摘要
采用HPLC-UV和LC-MS方法相结合测定分析了海柯皂苷元在蒺藜总皂苷灌胃Sprague-Dawley(SD)大鼠体内不同器官组织分布动态变化。结果表明在大鼠胃肠中蒺藜总皂苷中有部分海柯皂苷水解为海柯皂苷元;在从6到32 h灌胃蒺藜总皂苷的大鼠肝脏中可检测到海柯皂苷元的存在,证明有一定量海柯皂苷元从大鼠胃肠道吸收进入体内;对于海柯皂苷元检测,API-MS检测方法较UV方法有更高的灵敏度,且有好的专属性。
引用
收藏
页码:927 / 931
页数:5
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