454测序法在环境微生物生态研究中的应用

被引:15
作者
徐晓宇 [1 ]
刘和 [2 ]
机构
[1] 江南大学医药学院
[2] 江南大学环境与土木工程学院
关键词
454测序; 环境微生物生态; 宏基因组;
D O I
10.13560/j.cnki.biotech.bull.1985.2010.01.011
中图分类号
X172 [环境微生物学];
学科分类号
摘要
传统的Sanger测序技术虽已成熟,但其速度和成本的限制满足不了大规模测序的要求。第二代高通量测序技术结合了乳胶微粒和皮升级反应的454焦磷酸测序法,作为一种高通量测序技术,具有分析结果准确、高速、高灵敏度和高自动化的特点。对454测序法的技术原理和操作步骤进行了介绍,对近年来运用该方法在环境微生物生态研究领域的进展进行了综述。
引用
收藏
页码:73 / 77
页数:5
相关论文
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