肺癌多种基因同步检测及临床相关性(诊断及预后)的研究

被引:3
作者
孔令非
银平章
刘正国
郑晓芙
党伟一
王宝梅
赵跃武
机构
[1] 河南省人民医院病理科!河南郑州
[2] 郑州铁路局中心医院!河南郑州
关键词
非小细胞肺癌; p16; pRb; cdk4; cyclinD1; nm23H1; CD44v6;
D O I
暂无
中图分类号
R734.2 [肺肿瘤];
学科分类号
摘要
目的 :为检测非小细胞肺癌中多种基因的改变 ,并探讨其临床相关性 (诊断及预后 )。方法 :本研究用LSAB(labelledstreptavidinbiotin)法同步检测 10 4例非小细胞肺癌 (NSCLC)组织中抗癌基因p16,pRb及癌基因cy clinD1,cdk4和转移相关基因nm 2 3 H1,CD4 4v6表达。结果 :3 2 8%的鳞癌和 5 3 7%的腺癌不表达p16蛋白 ,腺癌明显高于鳞癌 (P <0 0 5 ) ;腺癌只有 14 6%完全不表达pRb蛋白 ,鳞癌不表达率为 3 6 2 % (P <0 0 5 ) ;5 7 7%的肿瘤只表达p16或pRb中的一项 ;3 2 7%的肿瘤两者都表达 ,但两者表达强度差异明显 ,9 6%的肿瘤两者均表达且表达强度一致 ;88 6%的肿瘤表达cdk4,在高分化鳞癌中表达率为 66 7% ,中分化鳞癌 96 3 % ,低分化鳞癌达10 0 % ,大约 70 %的肺癌中有cyclinD1表达 ,而正常粘膜中只有 2 0 %有表达 (P <0 0 0 0 1) ,表明非小细胞肺癌中cy clinD1的表达明显增高。在无淋巴结转移组 ,其nm 2 3的表达率高达 78 8% ,与有淋巴结转移组的表达率 2 1 9%相比 ,有非常显著性差异 (P <0 0 0 0 1) ;有淋巴结转移组 ,CD4 4v6的表达率高达 96 9% ,与无淋巴结转移的表达率相比 ,差异具非常显著性 (P <0 0 0 0 1)。结论 :①p16及pRb基因的表达在肺癌中明显减弱 ,均与非小细胞肺癌的组织学类型有关
引用
收藏
页码:31 / 35
页数:5
相关论文
共 9 条
[1]  
Expression of CD44 variant proteins in human colorectal cancer is related to tumor progression. Wielenga VJ,Heider KH,Offerhaus GJ,et al. Cancer Research . 1993
[2]  
Overexpression of nucleoside diphosphate/kinase A/nm23-H1 protein in human lung tumors: association with tumor progression in squamous carcinoma. Gazzeri S,Brambilla E,Negoescu A,et al. Laboratory Investigation . 1996
[3]  
Inversely correlated expression of p16 and Rb protein in non-small cell lung cancers: an immunohistochemical study. Sakaguchi M,Fujii Y,Hirabayashi H,et al. International Journal of Cancer .
[4]  
Genetic abnormalities in lung cancer and their prognostic implication. Mirsudomi T,Takahashi T. Gan To Kagaku Ryono . 1996
[5]  
Transcriptional repression of the D-type cyclin-dependent kinase inhibitor p16 by the retinoblastoma susceptibility gene product pRb. Li Y,Nichols MA,Shay JW,et al. Cancer Research . 1994
[6]  
Assignment of a locus for familial melanoma, MLM, to chromosome 9p13-p22. Cannon-albright LA,Goldgar DE,Meyer LJ,et al. Science . 1992
[7]  
Standard and variant CD44 isoforma are commonly expressed in lung cancer of the non-small cell type but not of the small cell type. Ariza A,Mate JL,Isamat M,et al. Journal of Paleopathology . 1995
[8]  
A cell cycle regulator potentially involved in genesis of many tumor types. Kamb A,Gruis NA,Weaver Feldhaus J,et al. Science . 1994
[9]  
A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4. Serrano M,Hannon GJ,Beach D,et al. Nature . 1993