提高植物游离蛋氨酸含量的基因工程途径

被引:5
作者
宋时奎 [1 ]
马凤鸣 [1 ]
韩天富 [2 ]
机构
[1] 东北农业大学农学院
[2] 中国农业科学院作物科学研究所
基金
北京市自然科学基金;
关键词
蛋氨酸; 胱硫醚γ-合成酶(CGS); S-腺苷甲硫氨酸,基因工程;
D O I
暂无
中图分类号
S336 [生物技术育种方法];
学科分类号
摘要
蛋氨酸是一种含硫的必需氨基酸,在人类和动物营养中占有重要地位,但很多粮食作物(如豆类)的蛋氨酸含量偏低,通过常规育种手段提高作物蛋氨酸含量的潜力有限。近年来,利用基因工程手段已成功地在许多植物中提高了蛋氨酸的含量。本文综述了通过基因工程手段提高植物蛋氨酸含量的两种途径:提高富含蛋氨酸的储藏蛋白含量和提高游离蛋氨酸的含量。文中重点介绍了蛋氨酸合成过程中的关键酶——胱硫醚γ-合成酶(CGS)的功能、胱硫醚γ-合成酶N端在维持CGS mRNA稳定性方面的作用和对其进行调控的分子机制,指出了目前通过分子育种手段提高蛋氨酸含量方面存在的问题,认为要提高豆类作物的含硫氨基酸含量,应将CGS基因和富含蛋氨酸的储藏蛋白基因同时进行转化。
引用
收藏
页码:849 / 854
页数:6
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