DR5在TRAIL诱导Jurkat细胞凋亡中的作用

被引：10

作者：

马远方

杨东亮

陆士新

陈有海

机构：

[1] 河南大学细胞与分子免疫学实验室

[2] 华中科技大学同济医学院免疫学研究所

[3] 中国医学科学院肿瘤研究所

[4] 宾夕法尼亚大学医学院病理与实验医学系开封

[5] 武汉

[6] 北京

[7] 美国费城

来源：

中国免疫学杂志 | 2004年 / 05期

关键词：

肿瘤坏死因子相关的凋亡诱导配体; 死亡受体5; 凋亡; 抗DR5单克隆抗体;

D O I：

暂无

中图分类号：

R73-36 [治疗实验];

学科分类号：

100214 ;

摘要：

目的 :研究DR5在介导TRAIL凋亡信号中的作用。方法 :用含人DR5细胞外全长结构域重组DR5免疫BALB C小鼠 ,制备抗DR5单克隆抗体 ;流式细胞仪检测Jurkat细胞表面DR5表达水平 ;采用TRAIL凋亡检测试剂盒 ,检测Jurkat细胞凋亡率及抗DR5单克隆抗体对TRAIL诱导细胞凋亡的阻断率。结果 :DR5在Jurkat细胞表面的表达率为 94 83% ,TRAIL和抗TRAIL单克隆抗体能够诱导Jurkat细胞凋亡 ,呈现明显的剂量相关性 ,TRAIL浓度在 5 0～ 10 0ng ml时 ,杀伤率达 90 %以上。预先用抗DR5单克隆抗体与Jurkat细胞作用后 ,TRAIL对Jurkat细胞的杀伤功能几乎完全被mAb所阻断 ,其平均阻断率达90 4 9%。结论 :DR5在TRAIL诱导Jurkat细胞凋亡中起着十分关键的作用。

引用

页码：332 / 334

页数：3

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