TGF-beta1 Transgenic Mouse Model of Thoracic Irradiation: Modulation of MMP-2 and MMP-9 in the Lung Tissue

被引：10

作者：

杨坤禹 ^{[1
]}

刘莉 ^{[1
]}

张涛 ^{[1
]}

伍钢 ^{[1
]}

Ruebe Claudia ^{[2
]}

Ruebe Christian ^{[2
]}

胡豫 ^{[3
]}

机构：

[1] Union Hospital Cancer Center, Tongji Medical College, Huazhong Science and Technology University, Wuhan , China

[2] Department of Radiation Oncology, Saarland University Hospital, Homburg/Saar , Germany

[3] Department of Hematology, Union Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan , China

来源：

华中科技大学学报(医学英德文版) | 2006年 / 03期

关键词：

D O I：

暂无

中图分类号：

R-332 [医用实验动物学];

学科分类号：

100402 [劳动卫生与环境卫生学];

摘要：

To investigate the effects of TGF-β1 on the two gelatinases (MMP-2 and MMP-9), and their roles in lung remodeling after irradiation-induced lung injury. Expressions of TGF-β1 were measured with western blot, and expressions of MMP-2 and MMP-9 were analyzed with zymography in a TGF-β1 transgenic mouse model after thoracic irradiation with 12 Gy. We found expressions of TGF-β1 in the lung from the transgenic mice were three folds as compared to those from control mice. With densitometrical analysis, we found a significant decrease in MMP-9 activity in lung homogenates from the transgenic mice as compared with those from non-transgenic control mice 8 weeks after sham-irradiation (relative MMP-9 activity: C: 1.000±0.1091; TG: 0.4772±0.470 (n=8, P<0.05). But MMP-2 was constitutively expressed in the lung homogenates from the transgenic mice as compared to those from control mice 8 weeks after sham-irradiation (relative MMP-2 activity 8 weeks after sham-irradiation: C: 1.000±0.1556, TG: 1.0075±0.1472). Eight weeks after thoracic irradiation with 12 Gy, we observed a significant increase of MMP-2 and MMP-9 activity in lung homogenates from both transgenic and normal mice. In TGF-β1 transgenic mice relative MMP-9 activity was increased to 1.5321±0.2217 folds 8 weeks after thoracic irradiation with 12 Gy as compared to those after sham-irradiation (1.000±0.2153), and relative MMP-2 activity was increased to 1.7142±0.4231 folds. Our results show that TGF-β1 itself down-regulates activity of MMP-9, thereby decreases ECM degradation in lungs of TGF-β1 transgenic mice. Also we find that ionizing irradiation upregulates both MMP-2 and MMP-9 activity. Over-expressions of MMP-9 and MMP-2 after lung irradiation are involved in the inflammatory response associated with radiation-induced lung injury, and maybe further in radiation-induced lung fibrosis.

引用

页码：301 / 304

页数：4