天麻素抑制脂多糖诱导的人脐静脉内皮细胞的自噬

被引：4

作者：

解亚男 ^{[1
]}

杨萍 ^{[1
]}

文飞 ^{[1
]}

孙林 ^{[2
]}

陈媛丽 ^{[1
]}

陆地 ^{[1
]}

机构：

[1] 昆明医科大学人体解剖学与组织学胚胎学系

[2] 昆明医科大学第二附属医院心内科

来源：

解剖学报 | 2014年 / 45卷 / 06期

关键词：

天麻素; 脂多糖; 自噬; 丹酰尸胺; 免疫印迹法; 反转录-聚合酶链反应; 人脐静脉内皮细胞;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

100806 [中药药理学];

摘要：

目的探讨天麻素(Gas)在脂多糖(LPS)诱导的人脐静脉内皮细胞(HUVECs)自噬中的作用。方法用不同浓度的Gas(5、10、20、50μmol/L)预处理HUVECs 1h,再加入1mg/L LPS共培养12 h后,分别提取细胞总蛋白及mRNA,采用Western blotting及RT-PCR检测Beclin-1、ATG5及LC3的表达变化;采用丹酰尸胺(MDC)染色检测细胞中自噬小体(autophagosome)的表达变化;采用MTT检测HUVECs生存率的变化。结果 LPS诱导HUVECs产生自噬;Gas预处理后自噬水平明显降低且呈剂量依赖性;与此同时细胞的生存率明显增加。结论 LPS诱导HUVECs产生自噬;Gas通过抑制由LPS诱导产生的自噬,提高HUVECs的生存率。

引用

页码：824 / 829

页数：6

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