PCR-DGGE技术在哺乳动物胃肠道微生物多样性及菌群结构研究中的应用

被引：3

作者：

葛铮 ^{[1
]}

赵晗旭 ^{[1
]}

高云航 ^{[1
]}

马红霞 ^{[1
]}

张福君 ^{[2
]}

机构：

[1] 吉林农业大学动物科技学院

[2] 吉林正方农牧股份有限公司

来源：

动物医学进展 | 2013年 / 34卷 / 12期

关键词：

基于PCR的变性梯度凝胶电泳(PCR-DGGE); 哺乳动物; 胃肠道微生物;

D O I：

10.16437/j.cnki.1007-5038.2013.12.015

中图分类号：

S852.6 [家畜微生物学（兽医病原微生物学）];

学科分类号：

090601 ;

摘要：

为了进一步开展对野生哺乳动物胃肠道微生态的相关研究,论文对基于聚合酶链反应的变性梯度凝胶电泳(PCR-DGGE)技术进行了概述,并着重介绍了其在哺乳动物胃肠道微生物多样性和菌群结构方面的研究进展,发现PCR-DGGE技术克服了传统方法对微生态菌群分析的缺点,能够更好地检测和分析哺乳动物胃肠道微生态。采用PCR-DGGE技术对野生哺乳动物哺乳动物进行研究,能够为野生动物研究与保护奠定基础。

引用

页码：183 / 185

页数：3

共 22 条

[1]

Grain-rich diets differently alter ruminal and colonic abundance of microbial populations and lipopolysaccharide in goats.[J].Barbara U. Metzler-Zebeli;Stephan Schmitz-Esser;Fenja Klevenhusen;Leopold Podstatzky-Lichtenstein;Martin Wagner;Qendrim Zebeli.Anaerobe.2013,

[2] Bacterial profile from caecal contents and soft faeces in growing rabbits given diets differing in soluble and insoluble fibre levels [J].

Rodriguez-Romero, Norelys ;

Abecia, Leticia ;

Fondevila, Manuel .

ANAEROBE, 2012, 18 (06) :602-607

[3]

Composition and diversity of Canidae fecal flora.[J].Honghai Zhang;Xianhai Cao;Lei Chen.Acta Ecologica Sinica.2012, 5

[4] Evaluation of different storage methods to characterize the fecal bacterial communities of captive western lowland gorillas (Gorilla gorilla gorilla) [J].

Vlckova, Klara ;

Mrazek, Jakub ;

Kopecny, Jan ;

Petrzelkova, Klara J. .

JOURNAL OF MICROBIOLOGICAL METHODS, 2012, 91 (01) :45-51

[5]

Effect of susceptibility to enterotoxigenic Escherichia coli F4 and of dietary tryptophan on gut microbiota diversity observed in healthy young pigs.[J].Stefano Messori;Paolo Trevisi;Aude Simongiovanni;Davide Priori;Paolo Bosi.Veterinary Microbiology.2012,

[6]

Longitudinal investigation of the age-related bacterial diversity in the feces of commercial pigs.[J].Hyeun Bum Kim;Klaudyna Borewicz;Bryan A. White;Randall S. Singer;Srinand Sreevatsan;Zheng Jin Tu;Richard E. Isaacson.Veterinary Microbiology.2011, 1

[7]

Archaeal community structure diversity in the rumen of faunated and defaunated sheep.[J].D.P. Morgavi;J.P. Jouany;C. Martin;M.J. Ranilla.International Congress Series.2006,

[8]

Molecular monitoring and characterization of the faecal microbiota of healthy dogs during fructan supplementation.[J].Tom Vanhoutte;Geert Huys;Evie De Brandt;George C. Fahey;Jean Swings.FEMS Microbiology Letters.2005, 1

[9]

藏猪肠道微生物多样性的研究.[D].肖文萍.西北农林科技大学.2012, 12

[10]

几种常用实验动物与人肠道主要菌群多样性比较.[D].李伟.西南大学.2011, 09

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