高分辨率溶解曲线分析技术在大豆Ecotilling中的应用

被引：19

作者：

焦丽 ^{[1
]}

刘相国 ^{[2
]}

杨春明 ^{[3
]}

王虎义 ^{[4
]}

郝东云 ^{[2
,4
]}

王庆钰 ^{[1
]}

机构：

[1] 吉林大学植物科学学院

[2] 吉林省农业科学院生物技术研究中心

[3] 吉林省农业科学院大豆研究中心

[4] 吉林大学生命科学学院

来源：

大豆科学 | 2011年 / 30卷 / 05期

关键词：

大豆; 高分辨率溶解曲线分析技术; Ecotilling; SNP;

D O I：

暂无

中图分类号：

S565.1 [大豆];

学科分类号：

090302 [植物营养学];

摘要：

将高分辨率溶解曲线(High Resolution Melting,HRM)技术运用在大豆Ecotilling中,对256份栽培大豆的大豆异黄酮合酶基因(IFS1)的外显子区域进行SNP筛查,并采用直接测序的方法对HRM检测结果进行验证。结果表明:模板均一化后的检测效果较未均一化的检测效果差异显著,野生型样本与待测样本按1∶1及1∶4比例混样溶解曲线阈值较高,其中1∶4混样适合高通量检测。HRM对IFS1基因外显子区域的检测结果与PCR产物测序的结果比对显示,对IFS1第5段外显子和第7段外显子检测的准确率分别达到91.2%和100%,表明该研究利用HRM技术建立的栽培大豆的Ecotilling技术体系是一种高灵敏度、高通量和低成本检测SNP的新方法。

引用

页码：843 / 846+850 +850

页数：5

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