通过农杆菌介导将番茄铁转运蛋白基因导入八棱海棠(英文)

被引：6

作者：

渠慎春

黄晓得

章镇

姚泉洪

陶建敏

乔玉山

张君毅

机构：

[1] 南京农业大学园艺学院,南京农业大学园艺学院,南京农业大学园艺学院,上海市农业科学院生物技术研究中心,南京农业大学园艺学院,南京农业大学园艺学院,南京农业大学园艺学院南京,南京,南京,上海,南京,南京,南京

来源：

植物生理与分子生物学学报 | 2005年 / 03期

关键词：

八棱海棠; 农杆菌; 番茄铁转运基因; 遗传转化;

D O I：

暂无

中图分类号：

S661.4 [海棠类];

学科分类号：

090201 ;

摘要：

用农杆菌介导法,成功地将番茄铁转运蛋白基因导入了苹果砧木八棱海棠。获得了19个卡那霉素抗性株系,其中有11个株系经PCR鉴定为阳性。Southern杂交结果显示:有9个转基因株系基因组中整合了完整的目的基因。选择其中含有单拷贝和3个拷贝目的基因的各一个株系进行水培试验,结果表明整合了单拷贝目的基因的转基因株系表现出较强的抗缺铁胁迫能力,5周后其植株的鲜重比对照高21%￣34%。

引用

页码：235 / 240

页数：6

共 8 条

[1]

Iron uptake, trafficking and homeostasis in plants[J] . Rüdiger Hell,Udo W. Stephan.Planta . 2003 (4)

[2]

Introduction of rol C into Marubakaidou[Malus prunifolia Borkh. var. ringo Asami Mo 84-A] apple rootstock via Agrobacterium tumefaciens[J] . Megumi Igarashi,Hiroyuki Ogasawara,Yoshimichi Hatsuyama,Akira Saito,Masahiko Suzuki.Plant Science . 2002 (3)

[3] Two iron-regulated cation transporters from tomato complement metal uptake-deficient yeast mutants [J].

Eckhardt, U ;

Marques, AM ;

Buckhout, TJ .

PLANT MOLECULAR BIOLOGY, 2001, 45 (04) :437-448

[4] Iron accumulation and enhanced growth in transgenic lettuce plants expressing the iron- binding protein ferritin [J].

Goto, F ;

Yoshihara, T ;

Saiki, H .

THEORETICAL AND APPLIED GENETICS, 2000, 100 (05) :658-664

[5]

Introduction of the reconstructed yeast ferric reductase gene, refre1, into tobacco[J] . Hiroyuki Oki,Hirotaka Yamaguchi,Hiromi Nakanishi,Satoshi Mori.Plant and Soil . 1999 (2)

[6]

Genetic transformation of the apple rootstock M26 with the Rol B gene and its influence on rooting[J] . Margareta Welander,Nathalie Pawlicki,Anna Holefors,Fiona Wilson.Journal of Plant Physiology . 1998 (3)

[7]

Investigation of Agrobacterium-mediated transformation of apple using green fluorescent protein: high transient expression and low stable transformation suggest that factors other than T-DNA transfer are rate-limiting[J] . Siela N. Maximova,Abhaya M. Dandekar,Mark J. Guiltinan.Plant Molecular Biology . 1998 (3)

[8]

The toxicity of antibiotics to plant cell cultures[J] . K. Pollock,D. G. Barfield,R. Shields.Plant Cell Reports . 1983 (1)

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