西兰花总黄酮的制备及体外抗肿瘤活性研究

被引：6

作者：

方秀兵 ^{[1
]}

黄颖丽 ^{[2
]}

徐国英 ^{[3
]}

张曼 ^{[3
]}

机构：

[1] 江西省彭泽县马当中心卫生院

[2] 广东省广州市朗圣药业有限公司

[3] 江西省九江学院基础医学院药学系

来源：

江西医药 | 2013年 / 48卷 / 05期

关键词：

西兰花; 黄酮; 大孔吸附树脂; 抗肿瘤;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

摘要：

目的探讨西兰花总黄酮的抗肿瘤活性。方法新鲜西兰花花蕾晒干粉碎后,用超声辅助70%的乙醇提取其中黄酮类成分,然后采用AB-8型大孔吸附树脂进行纯化,利用紫外扫描光谱法进行定量鉴定;采用MTT方法检测纯化前后西兰花黄酮类化合物对SMMC-7721细胞的生长抑制作用。结果使用超声辅助乙醇提取法西兰花黄酮得率为0.1%,粗提物纯度为2.63%,大孔吸附树脂纯化后纯度为9.28%。结论纯化后的西兰花总黄酮对SMMC7721细胞生长的抑制作用随剂量增加而增强,呈剂量依赖性。西兰花总黄酮可显著抑制肝癌细胞株SMMC-7721的生长。

引用

页码：397 / 399

页数：3

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