诺如病毒CHN02/LZ35666株RdRp和VP1基因序列分析

被引：9

作者：

靳淼 ^{[1
]}

樊景凤 ^{[2
]}

于天飞 ^{[1
]}

方肇寅 ^{[3
]}

王君伟 ^{[1
]}

机构：

[1] 东北农业大学动物医学院预防兽医学系

[2] 国家海洋环境监测中心

[3] 中国疾病预防控制中心病毒病预防控制所

来源：

病毒学报 | 2007年 / 01期

关键词：

诺如病毒; 病毒性胃肠炎; RdRp; 衣壳蛋白; 序列分析;

D O I：

10.13242/j.cnki.bingduxuebao.001785

中图分类号：

R373 [人体病毒学（致病病毒）];

学科分类号：

摘要：

Sequence analysis of a new norovirus(NV) isolated from Lanzou city of China was performed based on partial sequence of RNA dependent RNA polymerase(RdRp) and complete capsid protein(VP1) gene.The isolated strain CHN02/LZ35666 shared high sequence homology with GII-4 NVs.Nucleotide homologies of RdRp region and encoded capsid protein region were 90.4%-98.6% and 89.8%-95.7%,respectively,while amino acid homology of capsid protein region was 94.4%-97.4%.The analysis of GDD motif in RdRp region indicated this GDD motif of Lanzhou strain differed from those of the GII-4 predominant epidemic strains.Lanzhou strain formed an independent branch in GII-4 cluster in the phylogenetic tree based on nucleotide sequence of RdRp region and amino acid sequence of capsid protein.Sequence alignment revealed a mutation at the fourth key site of the receptor-binding interface in the strains isolated after 2002 compared with those of previous strains suggesting a possible change of binding pattern to HBGAs receptors.

引用

页码：63 / 67

页数：5

共 21 条

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