基因工程改良淀粉品质

被引：33

作者：

徐军望

李旭刚

朱祯

机构：

[1] 中国科学院遗传研究所!北京

来源：

生物技术通报 | 2000年 / 01期

关键词：

淀粉; 直链淀粉; 支链淀粉; 基因工程;

D O I：

10.13560/j.cnki.biotech.bull.1985.2000.01.003

中图分类号：

Q789 [基因工程的应用];

学科分类号：

摘要：

淀粉对人类生活十分重要，它不仅是人们的能量和营养来源，而且还是重要的工业原材料。对于淀粉合成过程及淀粉的加工、使用一直是淀粉研究的重点内容。淀粉的合成在最后阶段涉及到３个关键性的酶是：ＡＤＰＧ焦磷酸化酶、淀粉合成酶以及淀粉分支酶。它们分别催化ＡＤＰ－葡萄糖的形成、葡聚糖链的延伸以及分支链的形成。另外淀粉去分支酶对淀粉最终结构的形成也起到重要作用。本文将介绍上述４个酶近年来的生物化学和分子生物学研究进展，并在此基础上讨论利用基因工程改良淀粉品质的可能途径。

引用

页码：14 / 22

页数：9

共 43 条

[1] INHIBITION OF THE EXPRESSION OF THE GENE FOR GRANULE-BOUND STARCH SYNTHASE IN POTATO BY ANTISENSE CONSTRUCTS [J].

VISSER, RGF ;

SOMHORST, I ;

KUIPERS, GJ ;

RUYS, NJ ;

FEENSTRA, WJ ;

JACOBSEN, E .

MOLECULAR AND GENERAL GENETICS, 1991, 225 (02) :289-296

[2]

Comparison of proteins of ADP-glucose pyrophosphorylase from diverse sources[J] . Brian J. Smith-White,Jack Preiss. &nbspJournal of Molecular Evolution . 2004 (5)

[3] Introduction of sense and antisense cDNA for branching enzyme in the amylose-free potato mutant leads to physico-chemical changes in the starch [J].

Flipse, E ;

Suurs, L ;

Keetels, CJAM ;

Kossmann, J ;

Jacobsen, E ;

Visser, RGF .

PLANTA, 1996, 198 (03) :340-347

[4] MOLECULAR-CLONING AND CHARACTERIZATION OF NOVEL ISOFORMS OF POTATO ADP-GLUCOSE PYROPHOSPHORYLASE [J].

LACOGNATA, U ;

WILLMITZER, L ;

MULLERROBER, B .

MOLECULAR AND GENERAL GENETICS, 1995, 246 (05) :538-548

[5] ISOLATION AND CHARACTERIZATION OF A CDNA-ENCODING GRANULE-BOUND STARCH SYNTHASE IN CASSAVA (MANIHOT-ESCULENTA CRANTZ) AND ITS ANTISENSE EXPRESSION IN POTATO [J].

SALEHUZZAMAN, SNIM ;

JACOBSEN, E ;

VISSER, RGF .

PLANT MOLECULAR BIOLOGY, 1993, 23 (05) :947-962

[6]

The purification and characterisation of the two forms of soluble starch synthase from developing pea embryos[J] . Kay Denyer,Alison M. Smith. &nbspPlanta . 1992 (4)

[7] A CHLAMYDOMONAS-REINHARDTII LOW-STARCH MUTANT IS DEFECTIVE FOR 3-PHOSPHOGLYCERATE ACTIVATION AND ORTHOPHOSPHATE INHIBITION OF ADP-GLUCOSE PYROPHOSPHORYLASE [J].

BALL, S ;

MARIANNE, T ;

DIRICK, L ;

FRESNOY, M ;

DELRUE, B ;

DECQ, A .

PLANTA, 1991, 185 (01) :17-26

[8]

Complementation of the amylose-free starch mutant of potato （ Solanum tuberosum. ） by the gene encoding granule-bound starch synthase[J] . E. R. Leij,R. G. E. Visser,K. Oosterhaven,D. A. M. Kop,E. Jacobsen,W. J. Feenstra. &nbspTheoretical and Applied Genetics . 1991 (3)

[9]

Isolation of an amylose-free starch mutant of the potato （ Solanum tuberosum L.）[J] . J. H. M. Hovenkamp-Hermelink,E. Jacobsen,A. S. Ponstein,R. G. F. Visser,G. H. Vos-Scheperkeuter,E. W. Bijmolt,J. N. Vries,B. Witholt,W. J. Feenstra. &nbspTheoretical and Applied Genetics . 1987 (1)

[10]

The function of the Waxy locus in starch synthesis in maize endosperm[J] . Chia-Yin Tsai. &nbspBiochemical Genetics . 1974 (2)

← 1 2 3 4 5 →