左归丸促进小鼠未成熟卵母细胞体外核成熟的实验研究

被引：13

作者：

孙晓峰 ^{[1
]}

罗令 ^{[2
]}

孙启春 ^{[3
]}

高玉强 ^{[3
]}

贺又舜 ^{[1
]}

陈北阳 ^{[1
]}

吴霞 ^{[1
]}

机构：

[1] 湖南中医药大学

[2] 湖南省长沙市第八医院

[3] 山东省枣庄市市立医院

来源：

现代生物医学进展 | 2011年 / 11卷 / 03期

关键词：

左归丸; 血清; 卵母细胞; 生发泡破裂; 第一极体;

D O I：

10.13241/j.cnki.pmb.2011.03.007

中图分类号：

R285.5 [中药实验药理];

学科分类号：

1008 ;

摘要：

目的:研究左归丸对小鼠未成熟卵母细胞体外核成熟的影响。方法:制备左归丸含药血清,将生发泡(germinal vesicle,GV)期卵母细胞分别在不同采血时间获取的左归丸含药血清培养液中进行体外培养,观察左归丸含药血清对生发泡破裂(germinal vesicle breakdown,GVBD)和第一极体(the first polar body,PB1)排出的时效关系。结果:药物血清组卵母细胞GVBD的发生率高于正常血清组和对照组,于培养后4h差异最显著(P<0.01);药物血清组卵母细胞PB1的发生率高于正常血清组和对照组,于培养后18h差异最显著(P<0.01)。结论:2～2.5h左归丸含药血清对未成熟卵母细胞体外核成熟具有明显促进作用。

引用

页码：444 / 447

页数：4

共 24 条

[1]

In-vitro maturation of oocytes: biological aspects. A. Ali,M. Benkhalifa,P. Miron. Reproductive Biomedicine Online . 2006

[2]

Effect of duration of in vitro maturation on nuclear maturation and fertilizability of feline oocytes. M. Nagano,K. Uchikura,Y. Takahashi, et al. Theriogenology . 2008

[3]

Effects of estradiol-17b andprogesterone supplementation on in vitro nuclear maturation of canineoocytes. Kim MK,Fibrianto YH,Oh H J,et al. Theriogenology . 2005

[4]

Developmental Biology[C]. 2008年国际口腔及颅颌前沿研究研讨会,1600

[5]

Developmental Biology[C]. 2008年国际口腔及颅颌前沿研究研讨会,1600

[6]

Kinetics of gene expression andsignaling in bovine cumulus cells throughout IVM in differentmediums in relation to oocyte developmental competence,cumulusapoptosis and progesterone secretion. Salhab M,,Tosca L,Cabau C,et al. Theriogenology . 2011

[7]

Primate model of metaphaseI oocyte in vitro maturation and the effects of a novel glutathionedonor on maturation,fertilization,and blastocyst development. Curnow EC,Ryan JP,Douglas M,et al. Fertility and Sterility . 2010

[8] Oocyte nucleus controls progression through meiotic maturation [J].

Polanski, Z ;

Hoffmann, S ;

Tsurumi, C .

DEVELOPMENTAL BIOLOGY, 2005, 281 (02) :184-195

[9]

Kinetics of gene expression and signaling in bovine cumulus cells throughout IVM in different mediums in relation to oocyte developmental competence, cumulus apoptosis and progesterone secretion[J] . M. Salhab,L. Tosca,C. Cabau,P. Papillier,C. Perreau,J. Dupont,P. Mermillod,S. Uzbekova. &nbspTheriogenology . 2011 (1)

[10]

中药药效研究思路与方法[M]. 人民卫生出版社 , 陈奇主编, 2005

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