新疆棉花子叶节直接诱导多芽发生再生植株的研究

被引：10

作者：

孙立军 ^{[1
]}

郝秀英 ^{[2
]}

李雪源 ^{[3
]}

闫建庆 ^{[3
]}

王新勇 ^{[4
]}

毛鸿才 ^{[1
]}

狄月华 ^{[2
]}

机构：

[1] 新疆农业大学农学院

[2] 新疆农科院微生物所

[3] 新疆农科院经作所

[4] 新疆农科院吐鲁番长绒棉所

来源：

新疆农业科学 | 2006年 / 05期

关键词：

新疆棉花(陆地棉和长绒棉); 子叶节; 直接多芽诱导; 植株再生;

D O I：

暂无

中图分类号：

S562 [棉];

学科分类号：

0901 ;

摘要：

以中国三大棉花产区之一的新疆棉花中长绒陆地棉和特色长绒棉品种为试验材料,采用子叶节作外植体,为防止陆地棉品种外植体褐化的影响,以分步添加6-BA来启动和/或诱导多芽的发生,此时用葡萄糖作为碳源代替通常使用的蔗糖,经过伸长培养后,以1/2 MS培养基添加10 mg/L BA诱导生根,生根率可达72.2%。并通过对不同品种诱导多芽发生的比较,认为以子叶节为外植体直接诱导多芽发生受棉花品种的基因型影响不大,为用农杆菌介导法规模化遗传改良棉花奠定基础。

引用

页码：380 / 385

页数：6

共 24 条

[1] A simple and rapid Agrobacterium-mediated transformation protocol for cotton (Gossypium hirsutum L.):: Embryogenic calli as a source to generate large numbers of transgenic plants [J].

Leelavathi, S ;

Sunnichan, VG ;

Kumria, R ;

Vijaykanth, GP ;

Bhatnagar, RK ;

Reddy, VS .

PLANT CELL REPORTS, 2004, 22 (07) :465-470

[2] In vitro shoot regeneration from cotyledonary node explants of a multipurpose leguminous tree, Pterocarpus marsupium Roxb.. [J].

Chand, S ;

Singh, AK .

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2004, 40 (02) :167-170

[3] High-frequency somatic embryo production and maturation into normal plants in cotton (Gossypium hirsutum) through metabolic stress [J].

Kumria, R ;

Sunnichan, VG ;

Das, DK ;

Gupta, SK ;

Reddy, VS ;

Bhatnagar, RK ;

Leelavathi, S .

PLANT CELL REPORTS, 2003, 21 (07) :635-639

[4] Slow desiccation leads to high-frequency shoot recovery from transformed somatic embryos of cotton (Gossypium hirsutum L. cv. Coker 310 FR) [J].

Chaudhary, B ;

Kumar, S ;

Prasad, KVSK ;

Oinam, GS ;

Burma, PK ;

Pental, D .

PLANT CELL REPORTS, 2003, 21 (10) :955-960

[5]

Rapid plant regeneration from cotton ( Gossypium hirsutum L.).[J].Shengwei Zhu;Jingsan Sun.Chinese Science Bulletin.2000, 19

[6] Induction of somatic embryos by macrosalt stress from mature zygotic embryos of Panax ginseng [J].

Choi, YE ;

Yang, DC ;

Choi, KT .

PLANT CELL TISSUE AND ORGAN CULTURE, 1998, 52 (03) :177-181

[7] Phenol content, acidic peroxidase and IAA-oxidase during somatic embryogenesis in Theobroma cacao L. [J].

Ndoumou, DO ;

Ndzomo, GT ;

Niemenak, N .

BIOLOGIA PLANTARUM, 1997, 39 (03) :337-347

[8]

Micropropagation of mature Chinese tallow tree ( Sapium sebiferum Roxb.).[J].E. A. Siril;U. Dhar.Plant Cell Reports.1997, 9

[9]

In vitro induction of multiple shoots and plant regeneration in cotton ( Gossypium hirsutum L.).[J].D. C. Agrawal;A. K. Banerjee;R. R. Kolala;A. B. Dhage;A. V. Kulkarni;S. M. Nalawade;S. Hazra;K. V. Krishnamurthy.Plant Cell Reports.1997, 9

[10] Herbicide-resistant Acala and Coker cottons transformed with a native gene encoding mutant forms of acetohydroxyacid synthase [J].

Rajasekaran, K ;

Grula, JW ;

Hudspeth, RL ;

Pofelis, S ;

Anderson, DM .

MOLECULAR BREEDING, 1996, 2 (04) :307-319

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