甘蓝型油菜产量及其构成因素的QTL定位与分析

被引:26
作者
张书芬 [1 ]
傅廷栋 [1 ]
朱家成 [2 ]
王建平 [2 ]
文雁成 [2 ]
马朝芝 [1 ]
机构
[1] 华中农业大学作物遗传改良国家重点实验室
[2] 河南省农业科学院棉花油料作物研究所
基金
国家高技术研究发展计划(863计划);
关键词
甘蓝型油菜; 产量构成因素; 遗传图谱; 分子标记; QTL定位; 上位性;
D O I
暂无
中图分类号
S565.4 [油菜籽(芸薹)];
学科分类号
摘要
产量性状是复杂的数量性状,对种子的单株产量及其构成因素(全株总有效角果数、每角粒数、千粒重)进行QTL定位和上位性分析,确定其在染色体上的位置及其遗传效应,可以探讨油菜杂种优势产生原因,提高育种中对产量性状优良基因型选择的效率,达到提高油菜产量的目的。在双低油菜细胞质雄性不育保持系1141B和双高恢复系垦C1构建的F2作图群体中,运用SRAP、AFLP和SSR三种标记技术构建了一个甘蓝型油菜(Brassica napusL.)的分子标记遗传连锁图谱。共包含244个标记,分布于20个主要连锁群、1个三联体上,图谱总长度为2 769.5 cM。采用Windows QTLCartographer Version 2.0统计软件及复合区间作图法,对油菜单株产量及其3大构成因素进行QTL定位,共检测到QTLs 16个,分布在9个连锁群上,其中第6和13连锁群最多,均有3个。单个QTL解释性状表型变异的0.38%73.34%。对于同一性状,等位基因的增效作用既来自母本,亦源自父本;采用双向方差分析法对位点间互作及其上位性进行分析,检测到26对影响产量构成性状的上位性互作效应QTL,说明油菜基因组中存在大量控制产量的互作位点,油菜产量性状的上位性存在着多效性,上位性互作包括QTL与非QTL位点,其中以非QTL位点较多。一般互作位点的独立效应值较小,而互作的效应值显著增大,且一般超过两位点独立效应值之和。反映了控制产量性状基因的复杂性。上位性是甘蓝型油菜产量性状杂种优势的重要遗传基础。
引用
收藏
页码:1135 / 1142
页数:8
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