急性肺损伤对肺组织β-防御素-2基因表达的影响

被引：6

作者：

方向明

徐笑益

陈齐兴

舒强

机构：

[1] 浙江大学医学院附属邵逸夫医院麻醉科

[2] 浙江大学邵逸夫临床医学研究所

[3] 浙江大学医学院心胸外科

来源：

中华麻醉学杂志 | 2002年 / 05期

关键词：

防御素; 基因表达; 呼吸窘迫综合征,成人型;

D O I：

暂无

中图分类号：

R363 [病理生理学];

学科分类号：

摘要：

目的研究急性肺损伤（ALI）对肺组织β-防御素-2基因表达的影响。方法将60只雄性健康SD大鼠随机分成两组:对照组和急性肺损伤组,每组30只。5％苯巴比妥钠 120mg/kg腹腔注射麻醉,以LPS 200 μg/kg尾静脉注射复制急性肺损伤模型;对照组用等量生理盐水（NS）尾静脉注射。于尾静脉注射LPS或NS后0h、6h、12h、1d、3d、5d时杀死大鼠,剖胸取出肺组织。采用Trizol一步法提取肺组织总RNA,以GAPDH为内参,半定量RT-PCR检测肺组织β-防御素-2基因的表达水平,双脱氧末端终止法测序鉴定PCR产物的特异性。结果对照组0h时肺组织中β-防御素-2基因表达水平为 0.77± 0.14,各时间点肺组织中β-防御素-2基因表达水平无显著性变化（P>0.05）。ALI组6h时肺组织β-防御素-2基因的表达水平与对照组相比无显著性差异（P>0.05）,而12h、1d、3d、5d时显著增高（P<0.05）。结论 β-防御素-2基因在大鼠肺组织中有组成型表达,ALI上调肺组织中β防御素-2基因的表达。β-防御素-2基因的诱导性表达可能与ALI的发生发展有关。

引用

页码：35 / 38

页数：4

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