纯化、测定植物中细胞分裂素的免疫亲和层析法和酶联免疫法

被引:9
作者
杨中汉
陈良甫
机构
[1] 北京大学生物系
[2] 北京大学生物系 北京
[3] 北京
关键词
免疫亲和; 层析系统; 胚珠; 纤维素; 大孢子囊; 心皮; 细胞分裂素; 亲和柱; 植物;
D O I
10.13592/j.cnki.ppj.1993.01.021
中图分类号
学科分类号
摘要
<正> 近年来微量的植物激素的定量分析方法有很大发展,Weiler将放射免疫技术引入细胞分裂素的测定,许多研究者对植物激素的免疫测定做了改进。细胞分裂素的酶联免疫吸附分析(ELISA)具有灵敏度高、特异性强、不需要昂贵设备、无放射性污染等优点,日益为各实验室采用。如何简化植物样品的纯化步骤,提高检测的选择性和灵敏度仍然是细胞分裂素免疫测定的限制因素。本
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页码:56 / 59
页数:4
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