三七皂甙R1对HL-60细胞系体外诱导分化作用的初步研究

被引:22
作者
徐罗玲
王伯瑶
高军
机构
[1] 华西医大病理生理学教研室,华西医大钩端螺旋体病研究室,华西医大钩端螺旋体病研究室
关键词
三七皂甙; HL-60; 诱导分化;
D O I
暂无
中图分类号
学科分类号
摘要
光镜及电镜观察发现,经三七皂甙R180μg/ml处理 5日,可诱导68%的HL-60细胞分化,其中晚幼粒32%,杆状30%,分叶6%。证实了三七皂甙R2是HL-60细胞系的强诱导剂,可诱导HL-60细胞向中性粒细胞分化。3H-TdR掺入实验结果表明,三七皂甙R1在诱导HL-60细胞分化的同时,对细胞DNA和RNA的合成均有影响。与对照相比,48小时掺入抑制率3H-TdR为26.32%,3H-UR为18.57%(P<0.05);72小时掺入抑制率分别为17.46%和21.76%(P<0.05)。
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页码:124 / 127
页数:4
相关论文
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