抗虫玉米MON89034转化体特异性PCR检测技术研究

被引:8
作者
李飞武
李葱葱
刘娜
赵宁
邵改革
张明
机构
[1] 吉林省农业科学院/农业部转基因植物环境安全监督检验测试中心
关键词
转基因; 抗虫玉米; 转化体特异性; PCR检测;
D O I
10.13597/j.cnki.maize.science.2010.04.031
中图分类号
S513 [玉米(玉蜀黍)];
学科分类号
摘要
根据抗虫玉米MON89034外源插入片段5’端与植物基因组连接区序列设计特异性引物,并进行PCR扩增,预期产物大小为455 bp。以zSSIIb基因作为内标准基因,建立了转基因玉米MON89034转化体特异性定性PCR检测方法。对该方法进行重现性、特异性和灵敏度测试,结果表明:该方法能够特异性检测出MON89034转化体,以100 ngDNA为模板,该方法的检测灵敏度达到0.1%,约为40个起始模板拷贝。复合PCR检测结果还表明,在同一PCR反应管中可实现对zSSIIb基因和MON89034的同时检测。
引用
收藏
页码:40 / 44
页数:5
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