多溴联苯醚对大鼠肝细胞CYP1A2依赖性MROD活性的影响

被引：8

作者：

聂芳红 ^{[1
]}

陈进军 ^{[2
]}

Bunce Nigel ^{[3
]}

机构：

[1] 广东海洋大学食品科技学院

[2] 广东海洋大学动物医学系

[3] Dept．of Chemistry and Biochemistry,University of Guelph,Guelph,Ontario,NG

来源：

中国农学通报 | 2005年 / 12期

基金：

教育部留学回国人员科研启动基金;

关键词：

MROD分析法; CYP 1A2; 多溴联苯醚; 大鼠;

D O I：

暂无

中图分类号：

S865.12 [];

学科分类号：

摘要：

采用未成熟SD大鼠肝细胞微粒体为试验材料,以2,3,7,8-四氯-二苯基-并-二哑噁英(2,3,7,8- tetrachlorobenzo-p-dioxin,TCDD)作为参照,利用甲氧基-异酚噁脱甲基酶(methoxyresorufin-O- demethylase,MROD)竞争性抑制动力学和荧光光谱法分析法,测定了5种多溴联苯(polybrominated diphenyl ethers,PBDEs)对7-甲氧基-异酚噁唑脱甲基反应的影响,间接研究了这些PBDEs对CYP1A2 的竞争性抑制作用。结果表明,和TCDD相比,PBDEs对MROD活性的抑制作用较弱,亦即对CYP1A2的竞争性抑制作用较弱。

引用

页码：11 / 13

页数：3

共 12 条

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